摘要
目的为了解福建省禽类养殖业发达地区环境禽流感病毒的分布特征,对2021年12月至2023年12月地处山区的南平市禽类相关环境禽流感病毒分布开展调查。方法采集2021年12月至2023年12月南平市禽类相关环境样本,运用实时荧光定量RT-PCR法进行甲型流感病毒核酸检测,对阳性样本进行H3、H5、H7、H9和H10亚型鉴定。应用SPSS 26.0软件分析不同时间、禽类相关场所、样本类型禽流感病毒的分布特征和差异性。结果2021年12月至2023年12月,南平市禽类相关环境样本甲型流感病毒阳性率为49.16%(1435/2919),其中H3、H5、H9、H10亚型阳性率分别为0.72%(21/2919)、9.42%(275/2919)、33.20%(969/2919)、0.89%(26/2919),H7亚型未检出,混合型(含一种以上亚型)阳性率6.51%(190/2919),未分型(甲型流感病毒检测阳性但H3/5/7/9/10均为阴性)阳性率11.58%(338/2919)。甲型流感病毒阳性率高峰主要出现在秋冬季(9月至次年2月),活禽市场和屠宰场的甲型流感病毒、H9亚型、混合型以及未分型阳性率显著高于养殖场,禽饮用水、粪便样本的甲型流感病毒阳性率较高,阳性样本以H9亚型居多。结论南平市禽类相关环境中禽流感病毒阳性率以秋冬季为高;病毒在禽饮用水和粪便样本中的阳性率更高,并且在活禽市场与屠宰场等多种禽类聚集的场所呈现更高的多样性。
Objective To profile the distribution of avian influenza virus in poultry-related environment in poultry industry developed area in Fujian province,an investigation was conducted in Nanping city from Dec.2021 to Dec.2023.Methods The samples from multiple types of external environment related to poultry in Nanping city were collected from Dec.2021 to Dec.2023,and the real-time fluorescence quantitative RT-PCR used to detect and subtype the influenza A virus(FluA).SPSS 26.0 software was used to analyze the distribution characteristics of FluA in poultry-related environment and the differences in time,places and sample types.Results The overall positive rate of FluA in samples from poultry-related environment was 49.16%(1435/2919).The positive rates of H3,H5,H9 and H10 subtypes were 0.72%(21/2919),9.42%(275/2919),33.20%(969/2919),0.89%(26/2919)respectively,and no H7 subtype was detected.The positive rate of mixed type(more than one subtype of FluA detected in a same sample)was 6.51%(190/2919),and the positive rate of unknown subtype(positive for FluA but negative for H3/5/7/9/10)was 11.58%(338/2919).The higher positive rate of FluA mainly occurred in autumn-winter season(September to February of the following year).In live poultry markets and slaughterhouses,the positive rates of FluA,H9 subtype,mixed type and unknown subtype were significantly higher than that in poultry farms.The positive rate of FluA in poultry drinking water and feces was higher than samples of other types,most of the positive samples were H9 subtype.Conclusions The positive rate of FluA in poultry-related environment in Nanping city was higher in autumn-winter season.The investigation also showed that higher FluA positive rate in drinking water and feces sample and diversity of the virus existed in the place of multiple types of poultry clustered,such as live poultry markets and slaughterhouses.
作者
张雅婷
吴晶晶
林琦
翁育伟
Zhang Yating;Wu Jingjing;Lin Qi;Weng Yuwei(The School of Public Health,Fujian Medical University,Fuzhou 350122,China;Fujian Provincial Center for Disease Control and Prevention,Fujian Provincial Key Laboratory of Zoonosis Research,Fuzhou 350012,China)
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
2024年第2期138-143,共6页
Chinese Journal of Experimental and Clinical Virology
基金
福建省卫生健康中青年科研重大项目(2021ZQNZD006)
福建省自然科学基金项目(2021J01351)。
关键词
禽流感病毒
禽类相关环境
分布特征
Avian influenza virus
Poultry-related environment
Distribution characteristics
