摘要
为进一步明确市售食品动植物过敏原标注情况,该研究建立一种可同时检测21种动植物过敏原成分的多重连接依赖性探针扩增(MLPA)技术,并对其进行灵敏度实验,并结合实时荧光定量聚合酶链式反应(RT-fqPCR)检测技术对38种市售预包装食品中动植物过敏原成分进行比较分析。结果表明,采用MLPA检测技术可同时对21种动植物过敏原成分进行检测,扩增峰之间不存在交叉干扰,扩增峰实际大小和理论大小相差≤3 bp,检出最低脱氧核糖核酸(DNA)质量浓度为1 ng/μL;RT-fqPCR、MLPA技术检测标注过敏原成分食品的检出率分别为51.5%、44.1%,此外,MLPA检测法检出了6个标注可能含有过敏原成分样品中的过敏原成分,10个样品中未标记的过敏原成分。因此,采用MLPA技术检测21种动植物过敏原成分的灵敏度更高。
In order to further clarify the labeling of animal and plant-derived allergens in commercially available food,a multiplex ligation-dependent probe amplification(MLPA)technique was developed,which could simultaneously detect 21 animal-and plant-derived allergens in one reaction.The specificity and sensitivity of the newly established MLPA technique was analyzed.The animal-and plant-derived allergens in 38 commercially available pre-packaged foods were compared and analyzed using MLPA technique and real-time fluorescent quantitative polymerase chain reaction(RT-fqPCR)detection technology.The results showed that 21 species of animal and plant allergens could be detected by MLPA at the same time,there was no cross interference between amplification peaks,the difference between the actual and theoretical amplification peaks was less than 3 bp.The minimum detectable deoxyribonucleic acid(DNA)concentration was 1 ng/μl.The detection rates of RT-fqPCR and MLPA were 51.5%and 44.1%,respectively.In addition,the allergen components in 6 samples labeled with possible allergens and 10 samples with unlabeled allergens were detected by MLPA.Therefore,the sensitivity of MLPA technique to detect 21 animal and plant allergens was higher.
作者
何名扬
王鸣秋
刘艳
李诗瑶
朱必婷
张涛
郭雅晴
周陶鸿
彭青枝
HE Mingyang;WANG Mingqiu;LIU Yan;LI Shiyao;ZHU Biting;ZHANG Tao;GUO Yaqing;ZHOU Taohong;PENG Qingzhi(Hubei Provincial Institute for Food Supervision and Test,Key Laboratory of Detection Technology of Focus Chemical Hazards in Animal-derived Food for State Market Regulation,Hubei Provincial Engineering and Technology Research Center for Food Quality and Safety Test,Wuhan 430075,China)
出处
《中国酿造》
CAS
北大核心
2024年第5期255-260,共6页
China Brewing
基金
湖北省重点研发计划项目(2020BCA091)
湖北省食品质量安全监督检验研究院联合创新项目(HBQT-LH202102)。
关键词
动植物过敏原
多重连接依赖性探针扩增
实时荧光定量聚合酶链式反应
多重检测
animal-and plant-derived allergens
multiplex ligation-dependent probe amplification
real-time fluorescent quantitative polymerase chain reaction
multiple detection
