摘要
目的:观察肾炎止血丸对IgA肾病(IgAN)模型大鼠的治疗作用及其对lncRNA TUG1介导miR-320-5p调控PTEN/Cosmc通路的影响。方法:采用“牛血清白蛋白+四氯化碳+脂多糖”联用法构建IgAN大鼠模型,32只大鼠随机分为正常对照组、模型组、氯沙坦钾片组[20 mg/(kg·d)]、肾炎止血丸组[3.6 g/(kg·d)],每组8只,均连续给药6周。留取血液、肠黏膜及肾组织标本,分别应用ELISA法检测血清磷酸酶及张力蛋白同源基因(PTEN)、核心1β1,3半乳糖基转移酶伴侣蛋白(Cosmc)、半乳糖缺陷型IgA1(Gd-IgA1)水平;采用Real-time PCR、Western blot检测肠黏膜组织、肾脏组织lncRNA TUG1、miR-320、PTEN、Cosmc mRNA及蛋白表达。结果:①血清PTEN、Cosmc、Gd-IgA1:与模型组比较,各给药组PTEN、Cosmc升高明显(均P<0.01)、Gd-IgA1减少明显(P<0.01),与氯沙坦钾片组比较,肾炎止血丸PTEN、Cosmc升高及Gd-IgA1减少明显(均P<0.01)。②RT-PCR法检测肠黏膜组织、肾组织lncRNA TUG1、miR-320、PTEN、Cosmc mRNA表达:与模型组比较,氯沙坦钾片组肠黏膜组织及肾组织TUG1升高明显(均P<0.01),miR-320减少明显(P<0.01);与氯沙坦钾片组比较,肾炎止血丸肠黏膜组织及肾组织TUG1、PTEN升高明显,肠黏膜组织Cosmc升高明显(均P<0.01),肾组织miR-320减少明显(P<0.01)。③Western blot检测肠黏膜组织、肾组织PTEN、Cosmc蛋白表达:与模型组比较,各给药组肠黏膜组织PTEN蛋白表达升高(P<0.05),肾炎止血丸组Cosmc表达升高明显(P<0.05);与氯沙坦钾片组比较,肾炎止血丸PTEN升高有显著差异(P<0.05),Cosmc表达升高无显著差异(P>0.05)。各给药组肾组织Cosmc表达升高有极显著差异(P<0.01),与氯沙坦钾片组比较,肾炎止血丸肾组织PTEN升高有显著差异(P<0.05),Cosmc表达升高有极显著性差异(P<0.01)。结论:肾炎止血丸能够改善IgAN大鼠肾损伤;其作用机制可能与参与lncRNA TUG1介导miR-320调控PTEN/Cosmc通路有关。
Objective:To observe the therapeutic effects of Shenyan Zhixue Pills on rats with IgA ne-phropathy and explore its impact on the lncRNA TUG1-mediated miR-320 regulation of the PTEN/Cosmc pathway.Methods:IgA nephropathy(IgAN)rat models were constructed using a combination of bovine serum albumin,carbon tetrachloride,and lipopolysaccharide.32 rats were randomized into four groups:normal control,model,Losartan potassium[20 mg/(kg·d)],and Shenyan Zhixue Pills[3.6 g/(kg·d)],with 8 rats in each group and continuous administration for 6 weeks.Blood,intestinal mucosa and renal tissue specimens were retained,and serum PTEN,Cosmc,and Gd-IgA1 levels were measured by ELISA;The expressions of lncRNA TUG1,miR-320,PTEN,and Cosmc mRNA and protein in intestinal mucosal tissue and kidney tissue were determined by Real-time PCR and Western blot.Results:①Serum PTEN,Cosmc,Gd-IgA1:Compared with the model group,PTEN and Cosmc were elevated significantly(all P<0.01)and Gd-IgA1 was reduced significantly(P<0.01)in each administration group,and compared with the group of Losartan potassium tablets,PTEN and Cosmc were elevated and Gd-IgA1 was reduced significantly in the Shenyan Zhixue Pills group(all P<0.01).②RT-PCR method for the detection of intestinal mucosal tissue and renal tissue lncRNA TUG1,miR-320,PTEN,Cosmc mRNA expression:Compared with the model group,the intestinal mucosal tissue and renal tissue of the Losartan potassium tablet group TUG1 was elevated significantly(both P<0.01),miR-320 was reduced significantly(P<0.01),and compared with the Losartan potassium tablet group,the Shenyan Zhixue Pills intestinal TUG1 and PTEN of mucosal and renal tissues were elevated significantly,Cosmc of intestinal tissues was elevated significantly(both P<0.01),and miR-320 of renal tissues was reduced significantly(P<0.01).③Western blot detection of PTEN and Cosmc protein expression in intestinal mucosal tissue and kidney tissue:Compared with the model group,PTEN protein expression in intestinal mucosal tissue of each administered group
作者
陈瑶
张永刚
闫晓明
王海艳
李莲花
张佩青
陈明
CHEN Yao;ZHANG Yonggang;YAN Xiaoming;WANG Haiyan;LI Lianhua;ZHANG Peiqing;CHEN Ming(Heilongjiang Academy of Chinese Medicine Sciences,Harbin,Heilongjiang 150036;Qiqihar First Hospital,Qiqihar,Heilongjiang 161005)
出处
《中国中医药科技》
CAS
2024年第3期391-396,共6页
Chinese Journal of Traditional Medical Science and Technology
基金
黑龙江省中医药科研项目(ZHY2022-076)
黑龙江省自然科学基金资助项目(LH2021H070)。
