摘要
目的:采用单细胞测序的方法,分析SARS-CoV-2感染患者不同感染时期免疫组库特征,探讨其可能的发病机制。方法:利用NCBI数据库获得数据,设置对照组、进展组和康复组。使用RStudio、Origin、Hipliot和Excel软件对单细胞测序数据进行分析。结果:TCR在识别抗原和清除病毒的过程中发挥重要作用,免疫组库特征在不同感染时期有很大差异。细胞的克隆数量均超过26.92%。α链CDR3长度分布集中在14个氨基酸上,而β链集中在15个氨基酸上。V和J区基因片段使用频率不同,TRAV13-1、TRAJ20、TRBV20-1、TRBJ2-1等基因的使用频率差异有统计学意义(P<0.05)。进一步V-J基因组合分析显示,对照组和进展组、对照组和康复组的单链V-J对使用频率差异均有统计学意义(P<0.05)。对照组αβ双链V-J对使用频率最高的是TRAV19-J34-TRBV4-1-J2-1,进展组αβ双链V-J对使用频率最高的是TRAV12-1-J30-TRBV19-1-J2-1,康复组αβ双链V-J对使用频率最高的是TRAV12-2-J52-TRBV7-9-J1-5。结论:本研究分析免疫组库T细胞的全局特征,有助于理解SARSCoV-2感染患者的发病机制,使患者在感染初期及时得到有效的治疗。
Objective:Using single cell sequencing to analyze the characteristics of the immune repertoire of SARS-CoV-2 patients at different infection stages,and to explore the possible pathogenesis.Methods:Obtaining data in the NCBI database,and healthy control,progression and convalescence groups were set up.Analysis of single cell sequencing data by RStudio,Origin,Hipliot and Excel software.Results:TCR plays an important role in antigen recognition and virus clearance.Characteristics of the three immune repertoires are very different.The number of clones exceeded 26.92%.The length distribution ofαchain CDR3 was concentrated on 14 amino acids,whileβ-chain was concentrated on 15 amino acids.The frequency of gene fragments in V and J regions were different,and the frequency of TRAV13-1,TRAJ20,TRBV20-1 and TRBJ2-1 were statistically significant(P<0.05).Further V-J gene combination analysis showed that there were significant differences in the frequency of single chain V-J between the control group and the progression group,the control group and the rehabilitation group(P<0.05).The highest frequency ofαβdouble chain V-J in the control group was TRAV19-J34-TRBV4-1-J2-1,the highest frequency ofαβdouble-stranded V-J in the progression group was TRAV12-1-J30-TRBV19-1-J2-1,and the highest frequency ofαβdouble chain V-J in the convalescence group was TRAV12-2-J52-TRBV7-9-J1-5.Conclusion:This study analyzes the global characteristics of T cells in the immune repertoire,which is helpful to understand the pathogenesis of SARS-CoV-2 patients,timely and effective treatment of patients in the early stages of infection.
作者
宁可
高建龙
马恩泽
朱晓
NING Ke;GAO Jianlong;MA Enze;ZHU Xiao(School of Computer Science and Information Engineering,Harbin Normal University,Harbin 150025,China;School of Computer and Control Engineering,Yantai Univer-sity,Yantai 264005,China)
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2024年第5期1096-1101,共6页
Chinese Journal of Immunology
基金
国家自然科学基金(61902094)
黑龙江省自然科学基金(QC2018082)。
关键词
T细胞受体
免疫组库
感染时期
全局特征
TCR
Immune repertoire
Infected stages
Global characteristics
