摘要
目的:研究自组装类釉原蛋白两亲性寡肽的骨缺损修复作用。方法:构建具有黏附特性的自组装类釉原蛋白寡肽纳米凝胶、骨髓间充质干细胞(BMSCs)细胞膜片和大鼠股骨骨缺损模型,将SD大鼠随机分为空白对照(Control)组、BMSCs种子细胞膜片(Cell sheet)组、类釉原蛋白寡肽生物支架(OPA scaffold)组、BMSCs种子细胞膜片+类釉原蛋白寡肽生物支架(Cell sheet/OPA scaffold)组,将各组材料填充于股骨骨缺损处,在术后8周分别通过大体观察、微型CT扫描重建及组织学来评估骨缺损修复情况。结果:术后大体标本观察发现4组均有不同程度骨修复,Cell sheet组缺损区大致轮廓仍清晰可见且缺损区仍较深,骨修复最差。与Control组相比,其余两组骨修复较好,以Cell sheet/OPA scaffold组骨缺损区域修复最好,缺损轮廓已不明显。微型CT三维重建及定量分析结果显示,Cell sheet组新骨生成率低于Control组(P<0.05);OPA scaffold组和Cell sheet/OPA scaffold组新骨生成率优于Control组(P<0.05),两组缺损区域都有较高的骨小梁数量(Tb.N)、骨小梁厚度(Tb.Th)、相对骨体积(BV/TV)、连接密度(Conn.D)和较低的骨小梁分离度(Tb.Sp),其中以Cell sheet/OPA scaffold组最高。组织学结果显示,OPA scaffold组和Cell sheet/OPA scaffold组有较多趋向成熟的骨小梁生成,Control组和Cell sheet组有少量趋向成熟的骨小梁生成。结论:自组装类釉原蛋白寡肽纳米凝胶具有很好的成骨能力及骨缺损修复能力,具有作为骨组织工程支架材料的潜力加以进一步研究。
Objective:To investigate the reparative effect of self-assembled amelogenin-inspired oligopeptide amphiphile on bone defects.Methods:We prepared self-assembled amelogenin-inspired oligopeptide amphiphile nanogel with adhesive properties and bone marrow stromal cells(BMSCs)cell sheets,and constructed a rat femoral bone defect model.SD rats were randomly divided into four groups,blank control group(Control),BMSCs cell sheet group(Cell Sheet),amelogenin-inspired oligopeptide amphiphile scaffold group(OPA Scaffold),and BMSCs cell sheet plus amelogenin-inspired oligopeptide amphiphile scaffold group(Cell Sheet/OPA Scaffold).The femoral bone defects were filled with these materials,and the reparative effect was evaluated through gross observation,Micro-CT scanning and histopathological examination at week 8 after surgery.Results:Postoperative gross observation showed that varying degrees of bone repair occurred in all groups.The cell sheet group still had a clear outline of the defect area and the area was still deep,resulting in the worst bone repair.The other two groups exhibited better bone repair than the control group,and the cell sheet/OPA scaffold group had the best bone repair,and the defect area was no longer obvious.Micro-CT 3D reconstruction and quantitative analysis showed that the new bone formation rate in the cell sheet group was lower than that in the control group(P<0.05).The rates in the OPA scaffold group and the cell sheet/OPA scaffold group were higher than that in the control group(P<0.05),and both groups had higher trabeculae number,trabecular thickness,bone volume/total volume and connective density(Tb.N,Tb.Th,BV/TV and Conn.D)and lower trabecular spacing(Tb.Sp)in the defect area,with the cell sheet/OPA scaffold group having highest values.Histopathological examination showed that the OPA scaffold group and cell sheet/OPA scaffold group had more mature bone trabecular formation,while the control group and cell sheet group had fewer.Conclusion:Self-assembled amelogenin-inspired oligopeptide amphi
作者
罗菁菁
施敏
林家婷
LUO Jingjing;SHI Min;LIN Jiating(Stomatology Center,The First affiliated Hospital of Wannan Medical College,Wuhu 241001,Anhui,China)
出处
《皖南医学院学报》
CAS
2024年第2期113-117,共5页
Journal of Wannan Medical College
基金
皖南医学院中青年科研基金项目(WK2020F11)
安徽省卫生健康科研项目(AHWJ2023A10146)。
关键词
自组装
釉原蛋白
多肽
组织工程
细胞膜片
self-assembly
amelogenin
peptide
tissue engineering
cell sheet
