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男性不育患者人乳头瘤病毒感染与精液质量的相关性研究

Study on the relationship between human papillomavirus infection and semen quality in male infertile patients
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摘要 目的探讨人乳头瘤病毒(HPV)精液感染对不育男性精液常规参数、精浆生化及精子DNA损伤的影响。方法收集115例2023年5月至2023年12月就诊于武汉华科生殖专科医院男科门诊患者精液样本,采用荧光PCR法进行HPV基因分型检测以评估精液感染率,所有样本完成精液常规分析、精浆生化、精子DNA碎片指数(DFI)及DNA精子高着色性检测分析,比较感染组与未感染组精液质量各参数之间的差异。结果115例男科门诊患者精液样本中HPV检出11例,阳性率占9.57%;与HPV阴性组相比,HPV阳性组在精子总活力、前向运动精子百分率、精液白细胞浓度、精浆锌总量、精浆弹性蛋白酶浓度参数上有所降低,在精子DFI值上有所升高,且差异有统计学意义(P<0.05)。结论男性HPV精液感染会对精子运动能力、精液感染指标及精子DNA完整性产生影响,对男性专科门诊患者诊疗上应考虑HPV感染因素,同时对高危人群必要时进行HPV基因分型检测。 Objective To explore the effects of HPV semen infection on semen routine parameters,seminal plasma biochemistry and sperm DNA damage in infertile men.Methods Semen samples were collected 115 patients who were admitted to Wuhan Huake Hospital of Reproductive from May 2023 to December 2023.HPV genotyping was performed by fluorescent PCR method to evaluate semen infection rate.Semen routine analysis,semen plasma biochemistry,sperm DNA fragment index(DFI)and sperm high DNA stainability were performed.The differences of semen quality parameters between infected group and uninfected group were compared.Results Among the 115 semen samples,HPV was detected in 11 cases,with a positive rate of 9.57%.Compared with the HPV negative group,total sperm motility,percentage of forward motile sperm,semen leukocyte concentration,total seminal plasma zinc and seminal plasma elastase concentration significantly decreased in the HPV positive group,and sperm DFI increased.Conclusions HPV semen infection may affect sperm motility,semen infection index and sperm DNA integrity.The infection factors should be considered in the diagnosis and treatment of male infertility patients,and HPV genotyping should be performed in high-risk groups if necessary.
作者 田黎黎 曾树林 龚洁 陈永刚 潘利萍 叶臻 陈瑾 TIAN Lili;ZENG Shulin;GONG Jie;CHEN Yonggang;PAN Liping;YE Zhen;CHEN Jin(Wuhan Wuchang Hospital,China 2.Wuhan Center of Clinical Laboratory,Wuhan 430065,China;不详)
出处 《现代泌尿生殖肿瘤杂志》 2024年第2期100-103,108,共5页 Journal of Contemporary Urologic and Reproductive Oncology
基金 武汉市卫健委科研项目(WX21D61)
关键词 男性不育 人乳头瘤病毒 精液常规参数 精浆生化 Male infertility Human papillomavirus Semen routine parameters Seminal plasma biochemistry
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