摘要
为研究桑黄游离酚提取物体外降尿酸活性,以野生瓦宁木层孔菌、栽培粗毛纤孔菌、栽培瓦宁木层孔菌和野生粗毛纤孔菌4个不同来源的桑黄为研究对象,分析不同来源桑黄游离酚提取物对黄嘌呤氧化酶的抑制活性,及其在高尿酸细胞模型中的降尿酸作用。筛选出降尿酸活性强的桑黄游离酚提取物,并通过超高效液相色谱-串联质谱(ultra performance liquid chromatography/tandem mass spectrometry,UPLC-MS/MS)对其降尿酸活性物质进行分析。研究结果表明,栽培粗毛纤孔菌、栽培瓦宁木层孔菌和野生粗毛纤孔菌的游离酚提取物对黄嘌呤氧化酶具有显著的抑制作用(P<0.05),IC_(50)值分别为(94.63±2.73)μg/mL、(99.69±2.50)μg/mL和(106.32±5.06)μg/mL;栽培粗毛纤孔菌和野生瓦宁木层孔菌的游离酚提取物可显著抑制高尿酸细胞模型中尿酸生成(P<0.05)。进一步通过UPLC-MS/MS分析活性较强的栽培粗毛纤孔菌游离酚中主要成分发现,其黄酮类物质中金丝桃苷、表儿茶素、茶黄素、地奥司明及木犀草苷等,可能是其降尿酸的主要活性物质。该研究可为桑黄降尿酸产品的研究与开发提供部分理论参考。
This study aimed to study the uric acid(UA)lowering activity of Sanghuangporus sanghuang ethanol extract in vitro,the inhibitory activity of free phenol extracts of four different sources of Sanghuangporus sanghuang(wild Phellinus vaninii,cultivated Inonotus hispidus,cultivated Phellinus vaninii,and wild Inonotus hispidus)on xanthine oxidase was determined.Furthermore,the inhibitory effect of free phenol extracts on uric acid production in high uric acid cell models.Additionally,the main compounds in strong active extract were analyzed by UPLC-MS/MS.Results suggested that cultivated Inonotus hispidus,cultivated Phellinus vaninii,and wild Inonotus hispidus showed strong inhibitory effects on xanthine oxidase(XOD),the IC_(50)values were(94.63±2.73)μg/mL,(99.69±2.50)μg/mL,and(106.32±5.06)μg/mL,respectively.Cultivated Inonotus hispidus and wild Phellinus vaninii showed better UA lowering ability than those of wild Inonotus hispidus and cultivated Phellinus vaninii in the high UA cell model(P<0.05).Results obtained from UPLC-MS/MS showed that the strong active components of flavonoids including hyperoside,(-)-epicatechin,theaflavin,diosmin,and cynaroside,may be the active components for lowering uric acid.This study may provide some theoretical references for the application of uric acid-lowering products of Sanghuangporus sanghuang.
作者
赵克芳
肖阳
邢东旭
黎尔纳
杨琼
邹宇晓
庞道睿
廖森泰
ZHAO Kefang;XIAO Yang;XING Dongxu;LI Erna;YANG Qiong;ZOU Yuxiao;PANG Daorui;LIAO Sentai(College of Food Science and Technology,Guangdong Ocean University,Zhanjiang 524088,China;Sericultural&Agri-Food Research Institute,Guangdong Academy of Agricultural Sciences/Key Laboratory of Functional Food,Ministry of Agriculture and Rural Affairs/Guangdong Key Laboratory of Agricultural Products Processing/Maoming Branch,Guangdong Laboratory for Lingnan Modern Agriculture,Guangzhou 510610,China)
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2024年第10期119-126,共8页
Food and Fermentation Industries
基金
广东省重点领域研发计划项目(2020B020225005)
广东省农业科学院新兴学科团队建设项目(202119TD)
财政部和农业农村部:国家现代农业产业技术体系
2022年度高水平广东省农业科技示范市建设项目
广东省农业科学院地方分院建设项目(2022支撑01)。
关键词
桑黄
粗毛纤孔菌
黄酮
黄嘌呤氧化酶
降尿酸
Sanghuangporus sanghuang
Inonotus hispidus
flavonoids
xanthine oxidase
lowering uric acid
