摘要
目的:从柳蒿芽中分离、纯化多糖,对其抗氧化活性进行研究。方法:采用响应面法优化超声波辅助酶法提取柳蒿芽多糖的工艺条件。结果:最佳提取工艺为:超声功率300 W,料液比1∶30 g/mL,超声时间46 min,纤维素酶添加量2.0%,果胶酶添加量3.0%,此条件下多糖得率为7.67%。采用DEAE-52层析柱分离、纯化共得到6个多糖组分。对AIP-2和AIP-3两个组分进行抗氧化活性的研究表明,不同质量浓度AIP-2和AIP-3处理对H_(2)O_(2)诱导的HepG2细胞氧化损伤均有较强的浓度依赖性保护作用。与模型组相比,高质量浓度AIP-2和AIP-3(50μg/mL)处理使细胞存活率提高了54.35%和60.26%,SOD活力提高了35.86%和38.98%,CAT活力提高了173.02%和205.42%,GSH-PX活力提高了50.24%和50.78%,MDA含量降低了46.13%和53.87%。结论:柳蒿芽多糖具有良好的抗氧化活性。
Objective:Isolate and purify polysaccharides from Artemisia integrifolia and to investigate their antioxidant activity.Methods:The optimum extraction process was obtained by ultrasonic-assisted enzymatic extraction of polysaccharides from Artemisia integrifolia using response surface methodology.Results:The optimum extraction process was obtained as follows:ultrasonic power 300 W,material-liquid ratio 1∶30 g/mL,ultrasonic time 46 min,addition amount of cellulase 2.0%,addition amount of pectinase 3.0%,and under this condition,polysaccharide yield 7.67%.A total of six polysaccharide fractions were obtained by separation and purification using DEAE-52 chromatography column.Antioxidant activity studies on both AIP-2 and AIP-3 fractions showed that both AIP-2 and AIP-3 treatments at different mass concentrations had strong concentration-dependent protective effects against H_(2)O_(2)-induced oxidative damage in HepG2 cells.Compared with the model group,treatment with high mass concentrations of AIP-2 and AIP-3(50μg/mL)increased cell survival by 54.35%and 60.26%,SOD viability increased by 35.86%and 38.98%,CAT viability increased by 173.02%and 205.42%,GSH-PX viability increased by 50.24%and 50.78%,and MDA content decreased by 46.13%and 53.87%.Conclusion:Artemisia integrifolia polysaccharide has good antioxidant activity.
作者
季子琦
刘荣
樊梓鸾
Ji Ziqi;Liu Rong;Fan Ziluan(Northeast Forestry University,Harbin 150040)
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2024年第4期235-246,共12页
Journal of Chinese Institute Of Food Science and Technology
基金
中央高校科学前沿与交叉学科创新基金项目(2572019BA09)
黑龙江省自然科学基金联合引导项目(LH2020C035)。
关键词
柳蒿芽多糖
超声波辅助酶法提取
分离、纯化
HEPG2细胞
抗氧化
Artemisia integrifolia polysaccharide
ultrasonic-assisted enzymatic extraction
isolation and purification
HepG2 cells
antioxidant
