期刊文献+

布劳恩脂蛋白耐受对大肠杆菌感染诱导奶牛子宫内膜炎症反应的调控作用

Modulatory role of Braun lipoprotein tolerance on endometrial inflammatory response to Escherichia coli infection in dairy cows
原文传递
导出
摘要 子宫内膜炎是奶牛常见的生殖障碍疾病,大肠杆菌是引起该病的主要病原微生物。耐受是宿主的一种防御策略,可以减少感染对宿主适应性的负面影响。但布劳恩脂蛋白(Braun lipoprotein,BLP)耐受是否对大肠杆菌感染诱导的奶牛子宫内膜炎具有调控作用目前尚不清楚。本研究分析了BLP对大肠杆菌感染后子宫内膜上皮细胞和子宫内膜组织中炎症介质分泌的影响,以及BLP对子宫内膜组织中HMGB1的表达水平。此外,探究了BLP对子宫内膜上皮细胞中信号通路激活的影响。试验结果表明,BLP的存在可显著下调脂多糖(lipopolysaccharide,LPS)或大肠杆菌诱导的奶牛子宫内膜上皮细胞和子宫内膜组织中促炎性细胞因子的分泌水平,同时上调抗炎因子分泌水平。免疫荧光结果表明,BLP可显著降低奶牛子宫内膜组织中HMGB1的表达。值得注意的是,BLP的存在可显著下调LPS或大肠杆菌刺激诱导的MAPK p38和NF-κB p65的磷酸化水平。结果表明,BLP耐受在大肠杆菌诱导的子宫内膜炎中起着至关重要的作用,BLP耐受可显著降低奶牛子宫组织的炎症反应。 Endometritis is a common reproductive disorder in dairy cows,and E-scherichia coli(E.coli)is the main pathogenic microorganism causing the disease.Tolerance is a defensive strategy of the host to reduce the harmful effects of infection on the host's fitness.However,whether Braun lipoprotein(BLP)tolerance has a regulatory effect on E.coli infection-induced cow endometritis remains unclear.This study analyzed the effects of BLP on the secretion of inflammatory mediators in endometrial epithelial cells and endometrial tissues after E.coli infection,as well as the expression level of HMGB1 in endometrial tissues.In addition,the effects of BLP on the activation of signaling pathways in endometrial epithelial cells were investigated.The results showed that BLP could significantly down-regulate the secretion of pro-inflammatory cytokines and up-regulate the secretion of anti-inflammatory cytokines in the endometrial epithelial cells and endometrial tissues of dairy cows induced by LPS or E.coli,Immunofluorescence results showed that BLP could significantly reduce the expression of HMGB1 in cow endometrial tissue.Notably,BLP can significantly down-regulate the phosphorylation levels of MAPK p38 and NF-κB p65 induced by LPS or E.coli.The results indicated that BLP tolerance plays a crucial role in E.coli-induced endometritis,and BLP tolerance can significantly reduce the inflammatory response in cow uterine tissue.
作者 于琢雅 巩志国 任佩佩 赵佳敏 毛伟 张双翼 刘谕泽 吴敬泽 刘博 YU Zhuoya;GONG Zhiguo;REN Peipei;ZHAO Jiamin;MAO Wei;ZHANG Shuangyi;LIU Yuze;WU Jingze;LIU Bo(College of Veterinary Medicine,Inner Mongolia Agricultural University,Hohhot 010018,China)
出处 《中国兽医学报》 CAS CSCD 北大核心 2024年第2期356-362,共7页 Chinese Journal of Veterinary Science
基金 国家自然科学基金资助项目(32160854)。
关键词 耐受 奶牛子宫内膜炎 大肠杆菌 布劳恩脂蛋白 脂多糖 tolerance bovine endometritis Escherichia coli Braun lipoprotein lipopolysaccharide
  • 相关文献

参考文献2

二级参考文献15

  • 1王良娟,李跃民,马勋,周霞,王晓兰,秦学敏,王新华,魏学良.产后健康母牛与子宫炎牛子宫内细菌分离与鉴定[J].甘肃畜牧兽医,1996,26(2):11-12. 被引量:32
  • 2史远刚,吴颖慧,周乐,李键,王建辰.奶牛早孕诊断乳汁孕酮临界值的确定[J].黄牛杂志,1996,22(4):24-27. 被引量:11
  • 3威廉.C.雷布汉.奶牛疾病学[M].北京:中国农业出版社出版,1999. 被引量:9
  • 4明道绪.生物统计附试验设计[M].第三版.北京:中国业出版社,2003.320-322. 被引量:1
  • 5黄夺先.乳汁孕酮免疫测定法及其在母牛早期妊娠诊断上的应用.中国畜牧杂志,1987,(6):16-17. 被引量:3
  • 6陈家璞,主编.乳牛疾病学[M].第1版.北京:农业出版社,1992.239-279. 被引量:1
  • 7中国科学院微生物研究所《伯杰细菌鉴定手册》翻译组译.伯杰细菌鉴定手册[M].北京:科学出版社,1984. 被引量:2
  • 8汪成天,叶祝年,王云方.兽医实验检验手册[M].上海:上海科学技术出版社.1984.5-10. 被引量:2
  • 9Olson,J.D.;Ball,L.;Mortimer,R.G. et al. Aspects of bacteriology and endocrinology of cows with pyometra and retained fetal membrane[J].Am, J.Vet.Res, 1984, (45):2250-2255. 被引量:1
  • 10王世荣 庄文忠 郁心连.奶牛子宫内膜炎病原菌的检验.中国兽医科技,1993,14(1):6-8. 被引量:3

共引文献7

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部