摘要
以体外培养奶牛子宫内膜组织为研究对象,运用ELISA、荧光定量PCR、HE染色和免疫荧光法分析了内源性和外源性前列腺素D_(2)(prostaglandin D_(2),PGD_(2))在大肠杆菌(Escherichia coli,E.coli)感染奶牛子宫内膜组织过程中对细胞因子(IL-1β、IL-6、TNF-α)和损伤相关因子(HMGB-1)表达的调控作用。结果显示,与空白对照组相比较,E.coli感染组奶牛子宫内膜组织中COX-2和H-PGDS的基因表达显著升高(P<0.001);PGD_(2)分泌量也显著上升(P<0.001);内源性PGD_(2)抑制剂(H-PGDS inhibitor 1、HQL-79)能够显著抑制E.coli感染奶牛子宫内膜组织中促炎细胞因子(IL-1β、IL-6、TNF-α)的分泌、损伤相关因子(HMGB-1)的表达并减轻了组织损伤;外源性添加PGD_(2)后可显著促进E.coli感染奶牛子宫内膜组织中促炎细胞因子的分泌,HMGB-1的表达及组织损伤程度明显增加。结果表明,PGD_(2)能够通过调控E.coli感染奶牛子宫内膜组织的过程促炎细胞因子和损伤相关因子的表达,进而加剧了由细菌感染引起的组织损伤。
The aim of this study is to investigate the effect of endogenous and exogenous prostaglandin D2(PGD2)on the expression of cytokines(IL-1β,IL-6,TNF-α)and injury related factors(HMGB-1)in the endometrium of cows infected with Escherichia coli(E.coli).The results showed that compared with the blank control group,the gene expression of COX-2and H-PGDS in the endometrium of cows infected with E.coli was significantly increased(P<0.001).The secretion of PGD2also significantly increased(P<0.001).Endogenous PGD2inhibitors(H-PGDS Inhibitor 1,HQL-79)can significantly inhibit pro-inflammatory cytokines)in the endometrium of cows infected with E.coli(IL-1β,IL-6,TNF-α).The secretion and expression of injury related factors(HMGB-1)in mice reduced tissue damage.The exogenous addition of PGD2can significantly promote the secretion of pro-inflammatory cytokines in the endometrium of cows infected with E.coli,and significantly increase the expression of HMGB-1and the degree of tissue damage.The above results indicate that PGD2can regulate the expression of pro-inflammatory cytokines and injury related factors in the process of E.coli infection of bovine endometrial tissue,thereby exerting a regulatory effect on tissue damage caused by bacterial infection.
作者
郭莉莉
杨效林
龚鹏飞
王钰
钱英红
张双翼
何建文
刘博
毛伟
曹金山
GUO Lili;YANG Xiaolin;GONG Pengfei;WANG Yu;QIAN Yinghong;ZHANG Shangyi;HE Jianwen;LIU Bo;MAO Wei;CAO Jinshan(College of Veterinary Medicine,Inner Mongolia Agricultural University,Hohhot 010018,China;Key Laboratory of Clinical Diagnosis and Treatment Technology of Animal Diseases,Ministry of Agriculture and Rural Affairs,Hohhot 010018,China;Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences,Hohhot 010031,China;Huimin District Animal Epidemic Disease Prevention and Quarantine Center of Hohhot City,Hohhot 010030,China)
出处
《中国兽医学报》
CAS
CSCD
北大核心
2024年第4期728-734,共7页
Chinese Journal of Veterinary Science
基金
国家自然科学基金资助项目(32160851)
内蒙古自治区高等学校青年科技英才资助项目(NJYT22041)
内蒙古农业大学青年教师科研能力提升专项资助项目
内蒙古自治区自然科学基金资助项目(2022MS03048)。
