摘要
试验旨在快速检测犬巴贝斯虫抗体,利用胶体金免疫层析技术制备犬巴贝斯虫抗体胶体金检测卡并评价其功能性。将羊抗鼠免疫球蛋白G (IgG)和犬巴贝斯虫表面抗原Bg P47重组蛋白(Bg P47 Ag)包被于NC膜上分别做为质控线和检测线,胶体金标记的鼠IgG和鼠抗犬IgG均匀喷点在聚酯纤维膜上作为标记垫,将化学品配制的玻纤溶液均匀涂在样品垫上,并用吸水纸和PVC底板制备犬巴贝斯虫抗体胶体金检测卡,对其特异性、灵敏度、均一性、加速稳定性、实时稳定性进行评价,并与ELISA、PCR检测结果进行对比。结果显示,该检测卡不与犬莱姆抗体(lyme Ab)阳性犬血清、犬瘟热病毒抗体(CDV Ab)阳性犬血清、犬细小病毒抗体(CPV Ab)阳性犬血清、犬无形体抗体(Anaplasma Ab)阳性犬血清、犬三联疫苗阳性免疫犬血清样本发生交叉反应;可检测浓度稀释至1∶256倍的样本;有效期可达到24个月。研究表明,该检测卡可快速、准确地检测犬巴贝斯虫抗体,为动物临床诊断和治疗提供参考。
The purpose of the study was to rapidly detect Babesia canis antibody,and to prepare a colloidal gold detection card for Babesisia canis antibody by colloidal gold immunochromatography technology and evaluate its functionality.The goat anti-murine IgG and Bg P47 Ag were coated on NC membrane as quality control line and detection line,respectively.The colloidal gold-labeled murine IgG and murine anti-canine IgG were uniformly sprayed on the polyester fiber membrane as a labeling pad,the glass fiber solution of the chemical preparation was evenly applied to the sample pad,and the colloidal gold detection card for Babesia caninis antibody was prepared with absorbent paper and PVC bottom plate,and its specificity,sensitivity,uniformity,acceleration stability and real-time stability were evaluated,and the results were compared with the results of ELISA and PCR.The results showed that the test card did not cross-react with canine lyme antibody(lyme Ab)-positive canine serum,canine distemper virus antibody(CDV Ab)-positive canine serum,canine parvovirus antibody(CPV Ab)-positive canine serum,canine Anaplasma Ab-positive canine serum,and canine triple vaccine-positive immunized canine serum samples.Samples with concentrations diluted up to 1∶256 can be detected.It can be valid for up to 24 months.Studies have shown that the test card can quickly and accurately detect Babesia canis antibody,and provide a reference for clinical diagnosis and treatment of animals.
作者
王汝锦
孙晓笛
吴银河
Wang Rujin;Sun Xiaodi;Wu Yinhe(Hangzhou Alltest Biotechnology Co.Ltd.,Zhejiang Hangzhou 310018)
出处
《现代畜牧兽医》
2024年第3期7-11,共5页
Modern Journal of Animal Husbandry and Veterinary Medicine
关键词
犬巴贝斯虫病
犬巴贝斯虫抗体
胶体金检测卡
血液寄生虫病
Canine babesiosis
Babesia canis antibody
Colloidal gold detection card
Blood parasitic diseases
