摘要
【目的】探究丁酸梭菌(Clostridium butyricum)对花鲈(Lateolabrax maculatus)幼鱼生长性能,消化、免疫和抗氧化能力及肠道菌群的影响,为丁酸梭菌在花鲈鱼养殖中的应用提供理论依据。【方法】以花鲈幼鱼为试验材料设饲料中丁酸梭菌添加量为0(D0,对照)、0.25%(D1)、0.50%(D2)、1.00%(D3)、2.00%(D4)和4.00%(D5)6组,投喂花鲈幼鱼56 d,测定其生长性能、肠道消化酶活性、血清生化和免疫指标及肠道形态指标,分析肠道菌群组成。【结果】丁酸梭菌可显著降低饲料系数(P<0.05,下同),显著提高花鲈幼鱼的终末平均体质量,D3、D4和D5组的增重率及特定生长率均显著高于D0组。与D0组相比,D4组肠道糜蛋白酶和脂肪酶活性均显著提高,血清总胆固醇、甘油三酯含量和谷草转氨酶、谷丙转氨酶活性均显著降低。D4组溶菌酶、碱性磷酸酶和总一氧化氮合酶活性均显著高于D0组,D3组酚氧化酶活性显著高于D0组;与D0组相比,D3和D5组过氧化氢酶活性及总抗氧化能力均显著提高,D4组超氧化物歧化酶活性显著提高,丙二醛含量显著降低。D2、D3和D5组花鲈幼鱼肠道肌层厚及绒毛高度均显著高于D0组。D3组花鲈幼鱼肠道菌群OTU数量和Chao1指数均显著高于D0组,各试验组花鲈幼鱼肠道菌群Simpson指数和Shannon指数均显著高于D0组;在门分类水平上,丁酸梭菌降低了变形菌门和厚壁菌门相对丰度,提高了放线菌门、浮霉菌门、酸杆菌门、绿弯菌门、拟杆菌门和芽单胞菌门相对丰度;在属分类水平上,丁酸梭菌降低了劳尔氏菌属、肠弧菌属和弧菌属有害菌相对丰度,提高了节杆菌属和KD4-96_unclassified有益菌相对丰度。【结论】饲料中添加丁酸梭菌能显著提高花鲈幼鱼生长性能,提高消化、免疫和抗氧化能力,调节肠道菌群结构组成及其相对丰度,提高肠道菌群多样性,以2.00%添加量效果最好。
【Objective】This experiment was performed to investigate the effects of dietary Clostridium butyricum on growth performance,digestion,immunity,antioxidant capacity and intestinal microbiota of juvenile Lateolabrax macula‐tus,and provide a theoretical basis for the application of C.butyricum in the culture of L.maculatus.【Method】Juvenile L.maculatus was used as experiment material.Juvenile L.maculatus had been fed for 56 d with six diets containing 0(D0,control),0.25%(D1),0.50%(D2),1.00%(D3),2.00%(D4)and 4.00%(D5)C.butyricum.The growth performance,intestinal digestive enzyme activity,serum biochemical and immune indicators,and intestinal morphology indicators of juvenile L.maculatus were measured,and the composition of intestinal microbiota was analyzed.【Result】The dietary C.butyricum could significantly reduce the feed coefficient(P<0.05,the same below),significantly improved final mean body weight of L.maculates,the weight gain rate(WGR),and specific growth rate(SGR)in D3,D4 and D5 groups were significantly higher than the D0 group.Compared with D0 group,the activities of intestinal chymotrypsin,lipase were significantly increased in D4 group,while the contents of serum total cholesterol and triglyceride and the activities of glutamic oxalacetic transaminase and glutamic-pyruvic transaminase were significantly decreased.The activities of ly‐sozyme,alkaline phosphatase and total nitric oxide synthase in D4 group were significantly higher than those in D0 group,and the activities of phenoloxidase in D3 group were significantly higher than those in D0 group.Compared with D0 group,the catalase activity and total antioxidant capacity in D3 and D5 groups were significantly increased,the super‐oxide dismutase activity in D4 group was significantly increased,and the malondialdehyde content was significantly de‐creased.The intestinal muscle thickness and villus height in D2,D3 and D5 groups were significantly higher than those in D0 group.The OTU number and Chao1 index of the intestinal microflora of j
作者
徐创文
蒋魁
虞为
林黑着
王鹏飞
赵超
刘曦瑶
杨铿
XU Chuang-wen;JIANG Kui;YU Wei;LIN Hei-zhao;WANG Peng-fei;ZHAO Chao;LIU Xi-yao;YANG Keng(South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences/Key Laboratory of South China Sea Fishery Resources Exploitation&Utilization,Ministry of Agriculture and Rural Affairs/Guangdong Provincial Key Labo‐ratory of Fishery Ecology and Environment,Guangzhou,Guangdong 510300,China;Guangzhou Xinhailisheng Bio‐technology Co.,Ltd.,Guangzhou,Guangdong 510300,China;Sanya Tropical Fisheries Research Institute/Key Labo‐ratory of Efficient Utilization and Processing of Marine Fishery Resources of Hainan Province,Sanya,Hainan 572426,China;Shenzhen Base of South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Shen‐zhen,Guangdong 518121,China)
出处
《南方农业学报》
CAS
CSCD
北大核心
2024年第2期366-377,共12页
Journal of Southern Agriculture
基金
海南省科技专项(ZDYF2022XDNY349)
海南省自然科学基金项目(321QN0942)
中国水产科学研究院中央级公益性科研院所基本科研业务费专项(2023TD21)
深圳市科技计划(JCYJ20230807150859010)。
关键词
丁酸梭菌
花鲈
生长性能
免疫
消化
肠道菌群
Clostridium butyricum
Lateolabrax maculatus
growth performance
immunity
digestion
intestinal microbiota
