摘要
Polarity is a critical microenvironmental factor of the plasma membrane,which can offer valuable insights into various biological processes.Herein,we proposed a novel strategy for the construction of fluorescent agents to measure plasma membrane polarity by conjoining twisted intramolecular charge transfer(TICT)modulation and charge number control.It is shown that compounds with a stronger TICT tendency are more sensitive to polarity shifts due to the number of dialkylated amino groups present(from 1 to 3),and the molecules with two or more charged centers remain in the plasma membrane.Therefore,we developed two fluorescent agents with high polarity sensitivity,excellent turn-on ratios,and superior ability,to target the plasma membrane.In the wash-free fluorescence imaging and fluorescence lifetime tests,our designed agent could detect plasma membrane polarity with high precision,allowing effective distinction between cancer cells and normal cells based on their differences in plasma membrane polarity.Moreover,both fluorescence and fluorescence lifetime changes of the plasma membrane in the ferroptosis model established by Sorafenib confirmed an increase in plasma membrane polarity during cell ferroptosis.
基金
support of this work by the National Natural Science Foundation of China(grant nos.22067019 and 22367023)
Yunnan Provincial Science and Technology Department-Yunnan University Joint Special Project(grant no.202201BF070001-001)
the Postgraduate Research Innovation Foundation of Yunnan University(grant no.KC-22222295)
the Scientific Research Foundation Project of Yunnan Provincial Department of Education(grant no.2022Y028)
the National Research Foundation of Korea(CRI
project no.2018R1A3B1052702,J.S.K)
L.Yu thanks the China Scholarship Council(CSC
grant no.2021907030009).
