摘要
无患子(Sapindus mukorossi Gaertn)提取液常作为一种天然活性原料添加到洗护产品中,为推进无患子提取液在日化行业的应用,采用微生物发酵法对无患子水提液进行纯化,以多糖质量浓度和皂苷质量分数为指标,筛选出植物乳酸杆菌(Lactobacillus plantarum)作为发酵菌株,制得了无患子乳酸杆菌发酵液(SF)。从皮脂、炎症和致病菌的过度繁殖3个方面,研究了SF的抗痤疮功效。结果显示,质量浓度为20μg/mL的SF对SZ95细胞脂质合成率降低26.4%,对脂多糖(LPS)诱导的RAW 264.7细胞的NO、肿瘤坏死因子α(TNF-α)、白细胞介素-6(IL-6)和活性氧(ROS)表达量分别降低了52.3%、57.5%、56.2%和35.9%;采用二倍稀释法测定了SF对痤疮丙酸杆菌、糠秕马拉色菌和金黄色葡萄球菌的最小抑菌质量浓度分别为3.125、12.5和6.25 g/L;人体功效评价结果显示,受试者面部痤疮情况显著改善。
Sapindus mukorossi Gaertn extracts was often added into care products as a natural active raw material.In order to promote the application of Sapindus mukorossi Gaertn extracts in daily chemical industry,the water extract was purified by microbial fermentation method.Sapindus mukorossi fermentation liquid(SF)was then prepared using Lactobacillus plantarum as fermentation strain,which was selected based on polysaccharide mass concentration and saponin mass fraction,and evaluated for its anti-acne performance from the aspects of sebum,inflammation and overgrowth of pathogenic bacteria.The results showed that SF with a mass concentration of 20μg/mL led to a reduction in the lipid binding rate of SZ95 cells by 26.4%,and NO,tumor necrosis factor-α(TNF-α),interleukin-6(IL-6)and reactive oxygen(ROS)in RAW 264.7 cells induced by lipolyaccharide(LPS)by 52.3%,57.5%,56.2%and 35.9%,respectively.Moreover,the SF exhibited a minimum inhibitory mass concentration,which were measured by double dilution method,against Propionibacterium acnes,Malassezia furfur and Staphylococcus aureus of 3.125,12.5 and 6.25 g/L,respectively.Data from the human efficacy evaluation revealed that the facial acne situation was significantly improved.
作者
万凯波
陈殿松
马铃
常宽
王靖
WAN Kaibo;CHEN Diansong;MA Ling;CHANG Kuan;WANG Jing(Cosmetic Innovation Center,School of Chemical and Material Engineering,Jiangnan University,Wuxi 214122,Jiangsu,China;Adolf Research Innovation Laboratory,Guangzhou Degu Personal Care Products Co.,Ltd.,Guangzhou 510000,Guangdong,China)
出处
《精细化工》
EI
CAS
CSCD
北大核心
2024年第5期1076-1083,共8页
Fine Chemicals
关键词
无患子发酵液
痤疮
控油
抑菌
抗炎
医药原料
Sapindus mukorossi fermented liquid
acne
sebum control
bacteriostasis
anti-inflammatory
drug materials
