摘要
目的探讨急性呼吸窘迫综合征(ARDS)患儿血清长链非编码RNA OIP5-AS1(LncRNA OIP5-AS1)、微小RNA-218-5p(miR-218-5p)表达情况及二者在临床预后预测中的价值。方法回顾性选择2020年7月至2022年2月秦皇岛市第一医院收治的104例ARDS患儿为ARDS组。根据氧合指数(PaO_(2)/FiO_(2))将ARDS患儿分为重度亚组(PaO_(2)/FiO_(2)≤100 mmHg,n=32)、中度亚组(100 mmHg<PaO_(2)/FiO_(2)≤200 mmHg,n=38)、轻度亚组(200 mmHg<PaO_(2)/FiO_(2)≤300 mmHg,n=34)。根据ARDS患儿28 d院内死亡情况将其分为存活亚组(n=74)和死亡亚组(n=30)。另选择同期于本院进行健康体检的98例儿童作为对照组。比较各组血清LncRNA OIP5-AS1、miR-218-5p表达水平;收集患儿性别、年龄、气道峰值压、通气时间、入住ICU时间、动脉血二氧化碳分压(PaCO_(2))、动脉血氧分压(PaO_(2))、入院急性生理学与慢性健康状况评分系统Ⅱ(APACHEⅡ)评分、入院序贯器官功能衰竭评分(SOFA)、平均气道压、潮气量、白蛋白等资料。采用Pearson相关分析ARDS死亡患儿中血清LncRNA OIP5-AS1与miR-218-5p表达水平的相关性;绘制受试者工作特征(ROC)曲线分析血清LncRNA OIP5-AS1、miR-218-5p表达水平对ARDS患儿预后的预测价值;采用Logistic回归分析影响ARDS患儿死亡的因素。结果ARDS组血清LncRNA OIP5-AS1为2.04±0.38,显著高于对照组(1.01±0.21),miR-218-5p为0.45±0.16,显著低于对照组(1.02±0.20),差异均有统计学意义(P<0.05)。对照组、轻度亚组、中度亚组、重度亚组ARDS患儿血清LncRNA OIP5-AS1表达水平依次升高,miR-218-5p表达水平依次降低,差异均有统计学意义(P<0.05)。死亡亚组血清LncRNA OIP5-AS1表达水平及入院APACHEⅡ评分、入院SOFA、平均气道压、潮气量均高于存活亚组,miR-218-5p表达水平及PaO_(2)、白蛋白均低于存活亚组,差异均有统计学意义(P<0.05)。ARDS死亡患儿中血清LncRNA OIP5-AS1与miR-218-5p表达水平呈负相关(r=-0.503,P<0.05)。�
Objective To investigate the expression of serum long non-coding RNA OIP5-AS1(LncRNA OIP5-AS1) and microRNA-218-5p(miR-218-5p) in children with acute respiratory distress syndrome(ARDS) and their value in clinical prognosis prediction.Methods A total of 104 children with ARDS admitted to the First Hospital of Qinhuangdao from July 2020 to February 2022 were retrospectively selected as the ARDS group.According to the oxygenation index(PaO_(2)/FiO_(2)),children with RDS were divided into the severe subgroups(PaO_(2)/FiO_(2)≤100 mmHg,n=32),the moderate subgroups(100 mmHg<PaO_(2)/FiO_(2)≤200 mmHg,n=38),and the mild subgroups(200 mmHg<PaO_(2)/FiO_(2)≤300 mmHg,n=34).According to the in-hospital mortality rate of ARDS patients at 28 days,they were divided into the survival subgroup(n=74) and the death subgroup(n=30).Another 98 children who underwent health examinations in our hospital during the same period were selected as the control group.The expression levels of serum LncRNA OIP5-AS1 and miR-218-5p in each group were compared.The gender,age,peak airway pressure,ventilation time,ICU admission time,arterial carbon dioxide pressure(PaCO_(2)),arterial oxygen pressure(PaO_(2)),admission acute physiology and chronic health status evaluationⅡ(APACHE Ⅱ) score,admission sequential organ failure score(SOFA),mean airway pressure,tidal volume,albumin,and other data of the patient were collected.The correlation between serum LncRNA OIP5-AS1 and miR-218-5p expression levels in children with ARDS death were analyzed by Pearson correlation.The predictive value of serum LncRNA OIP5-AS1 and miR-218-5p expression levels on the prognosis of children with ARDS were analyzed by drawning receiver operating characteristic(ROC) curve;and the factors affecting the death of children with ARDS were analyzed by Logistic regression.Results The serum LncRNA OIP5-AS1 in the ARDS group was 2.04±0.38,which was significantly higher than that in the control group(1.01±0.21),the miR-218-5p in the ARDS group was 0.45±0.16,which was signific
作者
李书秀
闫梦洋
杜志云
LI Shu-xiu;YAN Meng-yang;DU Zhi-yun(Pediatric ICU,the First Hospital of Qinhuangdao,Qinhuangdao Hebei 066600,China;Department of Hand and Foot Surgery,the Second Hospital of Qinhuangdao City,Qinhuangdao Hebei 066600,China;Department of Neonatology,the First Hospital of Qinhuangdao,Qinhuangdao Hebei 066600,China)
出处
《临床和实验医学杂志》
2024年第7期706-710,共5页
Journal of Clinical and Experimental Medicine
基金
河北省医学科学研究课题计划(编号:20190849)
秦皇岛市科学技术研究与发展计划(编号:202101A128)。
