摘要
木薯(Manihot esculenta Crantz)作为热带地区第三大粮食作物,是世界近六亿人的口粮。木薯主要栽培于热带地区,常受到雨季水淹的危害,导致产量和品质严重下降,筛选耐水淹木薯种质具有重要意义。为研究不同木薯种质苗耐水淹性差异,采用盆栽模拟苗期水淹胁迫的方法,对水淹胁迫6 d下28份木薯种质的株高增长量、叶绿素相对含量、相对电导率和超氧化物歧化酶(superoxide dismutase,SOD)、过氧化物酶(peroxidase,POD)、过氧化氢酶(catalase,CAT)活性进行测定,采用变异系数分析、方差分析、相关性分析、主成分分析和隶属函数分析对其苗期耐水淹性进行综合评价。结果表明:28份木薯种质耐水淹性存在较大的差异,水淹胁迫显著抑制了15份种质生长(P<0.05),不同木薯种质对水淹胁迫的响应机制并不一致。相关性分析结果表明:株高增长量和叶绿素相对含量呈极显著正相关关系(P<0.01),相关系数为0.253;叶绿素相对含量和相对电导率呈极显著负相关关系(P<0.01),相关系数为0.266。主成分分析结果显示:前4个主成分可代替6个指标的82.85%贡献率,其中第I主成分的载荷主要是CAT,第II主成分的载荷主要是株高增长量,第III主成分的载荷主要是SOD,第IV主成分的载荷主要是叶绿素相对含量。通过隶属函数综合分析,28份木薯种质耐水淹性从强到弱依次为琼中1号、SC6068、SC201、ME191、M-F532、GR891、C222、GR3、F2000、罗勇80、46-12、GR5、KU58、H660、SC5、F03P、M-GR911、CH20、M-F671、COL713、老板2号、JG1301、GR911、E407、F274、SC8、M-SC5、泰国。本研究结果将为木薯耐水淹性新品种培育和耐水淹机理研究提供理论基础和材料支持。
Cassava(Manihot esculenta Crantz)is the third largest food crop in the tropics and is the food ration of nearly 600 million people in the world.Cassava is mainly cultivated in tropical regions,where often damaged by waterlogging in rainy season,which leads to serious decline in yield and quality.Therefore,it is important for selecting cassava germplasm resources with high waterlogging-resistance.In this study,a pot experiment was carried out,and plant height increment,relative chlorophyll content(SPAD),relative conductivity,superoxide dismutase(SOD),peroxidase(POD),and catalase(CAT)of 28 cassava germplasm resources were measured under waterlogging stress treatment after 6 days.Variance,correlation analysis,principal component analysis and subordinate function analysis were used to evaluate waterlogging resistance and screen the waterlogging tolerant germplasms at the seedlings stage.The waterlogging tolerance of 28 cassava germplasms had great difference and the response mechanism of different cassava germplasms to waterlogging stress showed huge difference among the 28 germplasm resources.There were significant positive correlation between plant height increment and relative chlorophyll content(P<0.01),and the correlation coefficient was 0.253.There were negative correlation coefficient between the relative chlorophyll content with relative conductivity of 0.266(P<0.01).Principal component analysis using 6 indexes indicated that the 4 principal components accounted for 82.85%of accumulative contribution rates among the germplasms.The main factors were CAT,plant height increment,SOD,and relative chlorophyll content,respectively.According to subordinate function analysis,waterlogging tolerance of the germplasms from high to low was Qiongzhong 1,SC6068,SC201,ME191,M-F532,GR891,C222,GR3,F2000,Luoyong 80,46-12,GR5,KU58,H660,SC5,F03P,M-GR911,CH20,M-F671,COL713,Boss 2,JG1301,GR911,E407,F274,SC8,M-SC5,Thailand.The results of this study would provide basic materials for the breeding of new varieties of cassava with high
作者
刘慧转
曹敏
唐丽
宋记明
李均一
冯亚亭
骆凯
陈银华
LIU Huizhuan;CAO Min;TANG Li;SONG Jiming;LI Junyi;FENG Yating;LUO Kai;CHEN Yinhua(Sanya Nanfan Research Institute,Hainan University,Sanya,Hainan 572025,China;College of Tropical Agrillulture and Forestry,Hainan University,Haikou,Hainan 570228,China;Agricultural Environment and Soil Research Institute,Hainan Academy of Agricultural Sciences,Haikou,Hainan 571100 China;Institute of Tropical and Subtropical Economic Crops,Yunnan Provincial Academy of Agricultural Sciences,Baoshan,Yunnan 678000,China)
出处
《热带作物学报》
CSCD
北大核心
2024年第4期691-702,共12页
Chinese Journal of Tropical Crops
基金
海南省自然科学基金项目(No.320QN193)
国家木薯产业体系生物防治与综合防控岗位科学家专项(No.CARS-11-hncyh)。
关键词
木薯
种质
耐水淹性
生理响应
隶属函数分析
cassava
germplasm
waterlogging tolerance
physiological response
subordinate function analysis
