摘要
【目的】分析不同程度的干旱胁迫处理对马棘生理指标的影响,探索其抗旱生理响应特征,为马棘抗旱种质筛选和遗传改良提供参考依据。【方法】以重庆地区广泛收集的36份野生马棘种质资源为试验材料,采用盆栽培养的方式待马棘幼苗生长至第90天时开始干旱处理,分别在胁迫0 d(对照组CK,土壤相对含水量为田间持水量的80%~85%)、胁迫7 d(中度干旱,土壤含水量为田间持水量的40%~50%)和胁迫14 d(重度干旱,土壤相对含水量为田间持水量的20%~25%)时测定各处理下马棘叶片的渗透调节物质含量、相对含水量和抗氧化酶活性,利用抗旱系数结合隶属函数和聚类分析法对马棘的抗旱性进行综合评价。【结果】在干旱胁迫下,36份马棘种质的生理响应变异丰富,不同种质的抗旱能力差异显著。通过对同一指标的均值比较分析发现,与对照相比,渗透调节物质丙二醛(MDA)、可溶性蛋白(SP)和脯氨酸(Pro)含量随着干旱程度增加而上升;超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性随着干旱程度增加而呈“凸”字形变化;相对含水量(RWC)随着干旱程度增加而降低。7个检测指标中,POD活性的变异系数最大,而RWC的变异系数最小。通过隶属函数、综合D值和聚类分析可将36份马棘种质的抗旱能力分为抗旱型(8份种质)和敏感型(28份种质)两类。通过对两种类型种质的生理指标分析发现,抗旱型种质的抗氧化酶活性和渗透调节物质Pro含量高于敏感型种质。【结论】干旱胁迫下36份马棘种质资源的生理响应具有丰富的多样性和变异,通过综合评价分析初步筛选出8份抗旱型马棘种质。
【Objective】The present paper aimed to analyze the impact of varying degrees of drought stress treatments on the physiological parameters of Indigofera pseudotinctoria,preliminarily evaluate the drought-resistant physiological responses of these materials,and provide a foundational reference for the selection and genetic improvement of drought-resistant germplasm in I.pseudotinctoria.【Method】36 wild I.pseudotinctoria germplasm resources collected in Chongqing area were used as experimental materials.Pot cultivation was used to start drought treatment when I.pseudotinctoria seedlings grew to the 90th day,and measure the content of osmoregulatory substances,relative water content,and antioxidant enzyme activity in the leaves of I.pseudotinctoria under 0 days of stress(control group CK,the relative soil water content was 80%-85%of the field water capacity),7 days of stress(moderate drought,soil relative moisture content was 40%-50%of the field water capacity),and 14 days of stress(severe drought,soil relative moisture content was 20%-25%of the field water capacity).Drought resistance coefficients,membership functions and cluster analysis were applied for a comprehensive evaluation of drought resistance.【Result】Under drought stress conditions,we observed a wide variation in physiological responses among the 36 I.pseudotinctoria germplasms,leading to significant differences in their drought resistance capabilities.Comparative analysis of mean values for various parameters showed that,in comparison to the control group,osmotic adjustment substances such as malondialdehyde(MDA),soluble protein(SP),and proline(Pro)increased with the severity of drought.The activities of superoxide dismutase(SOD),peroxidase(POD),and catalase(CAT)exhibited a‘convex’trend as drought severity intensified,while relative water content(RWC)decreased with increasing drought stress.Among the seven parameters measured,peroxidase(POD)activity showed the largest coefficient of variation,while relative water content(RWC)displayed the s
作者
陈静
何玮
徐远东
刘畅
马慧珍
孙万斌
范彦
CHEN Jing;HE Wei;XU Yuan-dong;LIU Chang;MA Hui-zhen;SUN Wan-bin;FAN Yan(Chongqing Academy of Animal Sciences/Chongqing Engineering Research Center for Grassland/Chongqing Crop Germplasm Rongchang Forage Resource Bank,Chongqing 402460,China;Qinghai University,Xining 810016,China)
出处
《西南农业学报》
CSCD
北大核心
2024年第3期542-551,共10页
Southwest China Journal of Agricultural Sciences
基金
重庆市科研机构绩效激励引导专项项目(cstc2021jxjl80017)
重庆市畜牧科学院畜牧科技创新团队培育项目(22535C23)
重庆市现代农业产业技术体系(CQMAITS202313)。
关键词
马棘
干旱胁迫
种质资源评价
抗旱性
生理响应
Indigofera pseudotinctoria
Drought stress
Evaluation of germplasm resource
Drought resistance
Physiological response
