摘要
【目的】分析抗枯萎病香蕉品种宿根连作根际土壤微生物群落结构特征变化,为深入研究抗枯萎病香蕉品种宿根连作抗性增强的作用机制及利用生防微生物进行香蕉枯萎病绿色防控提供理论依据。【方法】以抗枯萎病香蕉品种宝岛蕉为试验材料,在枯萎病发病严重土壤上宿根连续种植3造:组培苗第一代(第1造)、宿根第一代(第2造)和宿根第二代(第3造),调查各造枯萎病发病率,四分法采集各造土壤样本并测定土壤化学性质;采集第1造健康和发病植株、第2和第3造健康植株的根际土壤样本,分别编号为S1、V、S2和S3,重复3次,利用Illumina高通量测序平台对12个土壤样本的细菌16Sr RNA和真菌ITS区进行测序分析;采用样本复杂度(Alpha多样性指数)、主坐标(PCoA)和UPGMA聚类分析土壤样本细菌和真菌群落的丰富度和多样性、门和属水平上的群落构成及相对丰度差异;通过Spearman分析优势微生物群落与镰刀菌属的相关性。【结果】抗枯萎病香蕉品种宝岛蕉连续种植3造根际土壤化学性质未发生明显改变,种植第3造的香蕉枯萎病发病率显著低于第1和第2造(P<0.05)。随着种植年限的增加,根际土壤细菌群落的多样性整体呈逐年下降趋势,而真菌群落的多样性整体呈逐年上升趋势。连作改变了香蕉根际土壤微生物的群落构成,S2和S3细菌和真菌的群落结构相近,明显与S1和V群落结构分离。S2和S3的壶菌门(Chytridiomycota)的相对丰度较S1分别低88.02%和89.51%,Fungi_phy_Incertae_sedis的相对丰度分别高41.56%和82.81%,S3镰刀菌属(Fusarium)的相对丰度较S1、S2分别低45.60%和50.47%,与留芽种植宿根蕉抗性表现增强相关。发病植株根际土壤中变形菌门(Proteobacteria)和担子菌门(Basidiomycota)的相对丰度较高,而酸杆菌门(Acidobacteriota)的相对丰度较低。球囊菌门(Glomeromycota)、毛霉门(Mucoromycota)、隐真菌门(Rozellomycota)和壶菌�
【Objective】To analyze the changes in soil microbial community structure in the rhizosphere of banana varie-ties resistant to wilt disease through continuous cropping,providing a theoretical basis for in-depth research on the mechanism of enhanced resistance of banana varieties to wilt disease through continuous cropping and the use of biocon-trol microorganisms for green prevention and control of banana wilt disease.【Method】The fusarium wilt resistant banana variety Baodao banana was used as the experimental material,and the perennial roots were planted continuously for three generations on the soil with severe fusarium wilt disease:the first generation of tissue culture seedlings(the first genera-tion),the first generation of perennial roots(the second generation),and the second generation of perennial roots(the third generation).The incidence rate of fusarium wilt of each generation was investigated,and the soil samples of each generation were collected by quartering method and the chemical properties of the soil were determined.Collected rhizo-sphere soil samples from healthy and diseased plants of the first generation,as well as healthy plants of the second and third generations,numbered as S1,V,S2 and S3,and repeated 3 times.Used Illumina high-throughput sequencing plat-form to sequence and analyze the bacterial 16S rRNA and fungal ITS regions of 12 soil samples;utilized sample complexi-ty(Alpha diversity index),principal coordinates(PCoA),and UPGMA clustering analysis were used to analyze the rich-ness and diversity of bacterial and fungal communities in soil samples,as well as differences in community composition and relative abundance at the phylum and genus levels;analyzed the correlation between dominant microbial communities and the Fusarium through Spearman correlation analysis.【Result】The chemical properties of the rhizosphere soil of the banana variety Baodao banana with resistance to fusarium wilt did not change greatly after three successive generations of cultivation.The incidence rat
作者
赵明
苏祖祥
龙芳
邹瑜
莫天利
黄相
李凯
陈宇丰
盛静文
武鹏
ZHAO Ming;SU Zu-xiang;LONG Fang;ZOU Yu;MO Tian-li;HUANG Xiang;LI Kai;CHEN Yu-feng;SHENG Jing-wen;WU Peng(Bio-technology Research Institute,Guangxi Academy of Agricultural Sciences,Nanning,Guangxi 530007,China;Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Haikou,Hainan 571101,China)
出处
《南方农业学报》
CAS
CSCD
北大核心
2024年第1期1-12,共12页
Journal of Southern Agriculture
基金
广西科技重大专项(桂科AA22068090)
广西自然科学基金项目(2022GXNSFAA035543)
国家现代农业产业技术体系建设专项(CARS-31)
广西农业科学院科技发展基金项目(桂农科2024YP069)。
关键词
香蕉
抗枯萎病
宿根连作
根际土壤
微生物群落
banana
wilt disease resistance
continuous cropping
rhizosphere soil
microbial community
