摘要
目的探讨不同炎症程度慢性萎缩性胃炎(CAG)患者肠道菌群特征,为该类患者的治疗提供参考。方法对36例健康人(健康对照组)和8例轻度炎症(轻度炎症组)、26例中度炎症(中度炎症组)、8例重度炎症(重度炎症组)的CAG患者的粪便样本进行16S rRNA基因高通量测序(V4—V5区),采用Sobs、Shannon、Simpson、Ace、Chao指数评估4组对象肠道菌群的物种丰富度与多样性;采用PLS-DA分析评估4组对象肠道微生物群落结构;采用LDA与LEfSe分析确定4组对象相对丰度存在显著差异的肠道菌群;采用PICRUSt预测CAG患者显著富集肠道菌属的代谢通路信息。结果与健康对照组相比,轻、中、重度炎症组患者肠道菌群Sobs、Ace、Chao、Simpson指数有降低趋势,但差异均无统计学意义(均P>0.05)。Ruminococcus_gnavus_group、norank_f_Ruminococcaceae、Erysipelatoclostridium、Actinomyces在轻度炎症组中显著富集,Flavonifractor、Sellimonas在中度炎症组中显著富集,Eubacterium_rectale_group、Erysipelotrichaceae_UCG-003、Tyzzerella_3、Coprococcus_1、Candidatus_Soleaferrea在重度炎症组中显著富集。此外,Lachnospiraceae_UCG-004、Eubacterium_nodatum_group的丰度在健康对照组、轻度炎症组、中度炎症组、重度炎症组中呈递增趋势;Anaerostipes、Ruminiclostridium_9、Halomonas、Pelagibacterium的丰度在健康对照组、轻度炎症组、中度炎症组、重度炎症组中呈递减趋势,但差异均无统计学意义(均P>0.05)。PICRUSt分析结果显示4组对象肠道菌群代谢通路相似,但是代谢通路的丰度不同,其中重度炎症组患者肠道菌群代谢功能潜力高于健康对照组、轻度炎症组和中度炎症组。结论CAG患者肠道菌群变化的总体特征为产丁酸盐的菌属丰度减少,促炎菌属的丰度增加。促炎菌属可能通过附着于肠道上皮导致黏膜屏障受损和肠道通透性增加,影响宿主免疫反应,引起炎症。
Objective To observe the characteristics of gut microbiota in patients with chronic atrophic gastritis(CAG)with different degrees of inflammation,providing a reference for the treatment.Methods 16S rRNA gene high-throughput sequencing(V4-V5 region)was performed on fecal samples from 36 healthy subjects(health control group)and 8 CAG patients with mild inflammation(mild inflammation group),26 with moderate inflammation(moderate inflammation group),8 with severe inflammation(severe inflammation group).Sobs,Shannon,Simpson,Ace,and Chao indices were applied to assess the species richness and diversity;PLS-DA analysis was used to assess the microbial community structure;LDA and LEfSe analyses were used to identify the relative abundance of gut flora,and PICRUSt was used to predict the significantly enriched metabolic pathway of gut flora.Results Compared with the health control group,the Sobs,Ace,Chao,and Simpson indices tended to decrease in the mild,moderate,and severe inflammation groups,but the differences were not statistically significant(all P>0.05).Ruminococcus_gnavus_group,norank_f_Ruminococcaceae,Erysipelatoclostridium and Actinomyces were significantly enriched in the mild inflammation group;Flavonifractor and Sellimonas were significantly enriched in the moderate inflammation group;Eubacterium_rectale_group,Erysipelotrichaceae_UCG-003,Tyzzerella_3,Coprococcus_1 and Candidatus_Soleaferrea were significantly enriched in the severe inflammation group.In addition,the abundances of Lachnospiraceae_UCG-004 and Eubacterium_nodatum_group showed an increasing trend in health control,mild inflammation,moderate inflammation,and severe inflammation groups,while those of Anaerostipes,Ruminiclostridium_9,Halomonas and Pelagibacterium showed a decreasing trend in health control,mild inflammation,moderate inflammation and severe inflammation groups,but the differences were not statistically significant(all P>0.05).The PICRUSt results showed that the metabolic pathways in the four groups were similar,but the abundance valu
作者
郭本琼
刘怡
陈熠铧
杨德才
刘国萍
GUO Benqiong;LIU Yi;CHEN Yihua;YANG Decai;LIU Guoping(School of Traditional Chinese Medicine,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;不详)
出处
《中国微生态学杂志》
CAS
CSCD
北大核心
2024年第3期282-291,共10页
Chinese Journal of Microecology
基金
国家自然科学基金(81873236)
上海市卫健委面上项目(202340166)。
关键词
慢性萎缩性胃炎
肠道菌群
慢性炎症
肠黏膜屏障受损
Chronic atrophic gastritis
Gut microbiota
Chronic inflammation
Impaired intestinal mucosal barrier慢性萎缩性胃炎(chronic atrophic gastritis,CAG)
