摘要
通过超声波辅助低共熔溶剂提取蒲公英中的绿原酸。考察了低共融溶剂摩尔比[n(氯化胆碱)∶n(柠檬酸)]、液料比、超声功率、提取温度、超声时间对蒲公英绿原酸得率的影响。在单因素实验的基础上,通过响应面实验优化得出提取蒲公英绿原酸的最佳工艺条件为:低共熔溶剂含水量60%,n(氯化胆碱)∶n(柠檬酸)=1∶2,提取温度70℃,液料比35∶1(m L/g),超声功率300 W,超声时间42 min。在此条件下,蒲公英绿原酸的得率为2.86%±0.05%,与预测值接近2.89%接近,表明该模型可靠。同时对蒲公英绿原酸提取物抗氧化活性实验研究结果表明,蒲公英绿原酸的提取液在1~6μg/mL,DPPH自由基及ABTS+自由基的清除能力均随着浓度的增加而增大。在提取液质量浓度为6μg/mL时,两者的清除率分别为82.9%和84.2%,说明蒲公英绿原酸提取物具有较强的抗氧化活性。
Chlorogenic acid was extracted from dandelion by ultrasonic-assisted low eutectic solvent.The effects of low eutectic solvent molar ratio(choline chloride:citric acid),liquid-solid ratio,ultrasonic power,extraction temperature,and ultrasonic time on the yield of chlorogenic acid were investigated.Based on the single factor experiment,the optimal extraction conditions of chlorogenic acid from dandelion were obtained by response surface experiment:the low eutectic solvent with water content of 60%,the molar ratio of choline chloride and citric acid was 1:2,the extraction temperature was 70°C,the liquid-solid ratio was 35:1(mL/g),ultrasonic power was 300 W,and ultrasonic time was 42 min.Under these conditions,the yield of chlorogenic acid from dandelion was 2.86%±0.05%,which was close to the predicted value of 2.89%,indicating that the model was reliable.At the same time,the antioxidant activity of chlorogenic acid extract from dandelion was studied.The results showed that the extraction of chlorogenic acid from dandelion was in the range of 1~6μg/mL,and the scavenging ability of DPPH free radical and ABTS+free radical increased with the increase of concentration.When the extraction concentration was 6μg/mL,the scavenging rates of both were 82.9%and 84.2%,indicating that the chlorogenic acid extract from dandelion had strong antioxidant activity.
作者
卢娟
韦新银
谭春婷
罗清淋
王宏
吴春燕
Lu Juan;Wei Xinyin;Tan Chunting;Luo Qinglin;Wang Hong;Wu Chunyan(College of Chemistry and Biological Engineering,Guangxi Minzu Normal University,Chongzuo Guangxi 532200,China)
出处
《山西化工》
CAS
2024年第4期3-6,共4页
Shanxi Chemical Industry
基金
2023年自治区级大学生创新创业训练立项项目(S202310604039)
2020年度广西民族师范学院科学研究项目人才科研启动项目(2020FG002、2020FG004)。
关键词
蒲公英
绿原酸
低共融溶剂
超声波
抗氧化活性
dandelion
chlorogenic acid
low eutectic solvent
ultrasonic
antioxidant activity
