摘要
目的探讨蛇伤胶囊对竹叶青蛇伤miR-155/Toll样受体4(TLR4)/髓样分化因子88(MyD88)信号通路及炎症的影响。方法将18只雄性新西兰大白兔分为对照组、模型组和蛇伤胶囊组,每组6只。对照组正常饮食饮水,另两组注射7.5 mg/kg竹叶青蛇毒液6 h后,模型组灌胃348 mg/(kg·d)生理盐水,蛇伤胶囊组灌胃348 mg/(kg·d)蛇伤胶囊,均连续灌胃1周。观察实验兔的一般行为学,qRT-PCR检测外周血中miR-155a-5p、TLR4和MyD88表达,ELISA检测血清中白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)、γ干扰素(IFN-γ)和白细胞介素-4(IL-4)含量。结果与对照组比较,模型组精神和食欲变差,排便稀软带血,伤口溃烂,miR-155-5p、TLR4 mRNA、MyD88 mRNA、IL-6和TNF-α表达均显著增加(均P<0.01),IFN-γ和IL-4表达显著下降(均P<0.01)。蛇伤胶囊组较模型组情况明显好转,miR-155a-5p、TLR4 mRNA、MyD88 mRNA、IL-6和TNF-α表达显著下降(均P<0.01),IFN-γ和IL-4表达显著增加(均P<0.01)。结论蛇伤胶囊抑制竹叶青蛇伤造成的炎症反应,维持Th1/Th2细胞平衡,其作用机制可能与抑制miR-155/TLR4/MyD88轴的表达有关。
Objective To investigate the effects of Sheshang capsule on miR-155/Toll-like receptor 4(TLR4)/myeloid differentiation factor 88(MyD88)signaling pathway and the inflammation in Trimeresurus stejnegeri bites.Methods The 18 male New Zealand white rabbits were divided into control group,model group and Sheshang capsule group with 6 rabbits in each group.The rabbits in control group accepted normal diet and water.The model group was given intragastric infusion with 348 mg/(kg·d)normal saline and Sheshang capsule group was given intragastric infusion with 348 mg/(kg·d)Sheshang capsule for continous 7 days at 6 h after the infusion of 7.5 mg/kg Trimeresurus stejnegeri venom.The expressions of miR-146a-5p,TLR4 and MyD88 in peripheral blood were detected by reverse transcription quantitative real-time polymerase chain reaction(qRT-PCR),and the contents of interleukin-6(IL-6),tumor necrosis factor-α(TNF-α),interferon-γ(IFN-γ),and interleukin-4(IL-4)in serum were detected by enzyme-linked immunosorbent assay(ELISA).Results Compared with the control group,the spirit and appetite of the rabbits in model group became worse,the defecation was soft and bleeding,the wound festered,and the expressions of miR-155-5p,TLR4 mRNA,MyD88 mRNA,IL-6 and TNF-α were significantly increased(all P<0.01),and the expressions of IFN-γ and IL-4 were significantly decreased(all P<0.01).The rabbits in the Sheshang capsule group were significantly improved compared with the model group,and the expressions of miR-155-5p,TLR4 mRNA,MyD88 mRNA,IL-6 and TNF-αwere significantly decreased(all P<0.01),while the expressions of IFN-γand IL-4 were significantly increased(all P<0.01).Conclusions Sheshang capsule can inhibit the inflammatory response caused by Trimeresurus stejnegeri venom and maintain the balance of Th1/Th2 cells,and its mechanism may be related to the inhibition of miR-155/TLR4/MyD88 axis expression.
作者
王世军
沈芳华
张美吉
詹雪晶
王友前
蔡佳清
林奕丽
Wang Shijun;Shen Fanghua;Zhang Meiji;Zhan Xuejing;Wang Youqian;Cai Jiaqing;Lin Yili(The Affiliated People's Hospital of Fujian University of Traditional Chinese Medicine,Fuzhou 350004,China)
出处
《中国急救医学》
CAS
CSCD
2024年第5期397-401,共5页
Chinese Journal of Critical Care Medicine
基金
福建省卫生健康科技计划项目(2020GGA063)
福建省自然科学基金项目(2021J01895)
福建中医药大学校管优势学科课题(X2022023-专项)。
