摘要
目的:为了获得紫丁香蘑多糖的最佳提取工艺条件和质量检测方法,对水提醇沉法提取紫丁香蘑多糖的工艺进行优化,并采用多种色谱方法对紫丁香蘑多糖进行质量分析。方法:采用正交实验对提取工艺进行优化,柱层析法分离纯化,利用UV、HPLC、IR、HPCE图谱分析方法对紫丁香蘑多糖进行质量分析。结果:紫丁香蘑多糖提取的最佳提取条件为100℃,处理时间10h,料液比1∶25;柱层析法分离得到Ⅰ、Ⅱ两种组分,Ⅰ为主要组分;UV结果表明紫丁香蘑多糖中几乎不含核酸和蛋白质;HPLC结果表明Ⅰ组分有显著的多糖吸收峰;IR结果表明,在400~4000cm-1波数内有多处典型的多糖特征吸收峰,证明该多糖是由多种糖苷键相连接的杂多糖;HPCE结果显示紫丁香菇多糖是由氨基葡萄糖、葡萄糖醛酸等6种单糖组成。结论:采用UV、HPLC、IR、HPCE等色谱手段对紫丁香蘑多糖的结构和性质进行了研究,其成果对开发新的紫丁香蘑多糖保健食品和药品,具有重要的指导意义。
Objective:In order to obtain the optimal extraction process conditions and quality detection method for polysaccharides from Lepista nuda.To investigate the optimum extraction process of polysaccharides from Lepista Sordida Sing and to analyse the quality of polysaccharides by using a variety of chromatographic methods.Methods:Orthogonal method to optimize extraction process,Column chromatography purification,UV、HPLC、IR、HPCE Spectra Analysis.Results:Opimal process of extraction procedure of polysaccharides from Lepista Sordida Sing were obtained:temperature 100℃、extraction time 10h、material liquid ratio 1∶25;two componentsⅠandⅡwas separated by Column chromatography,Ⅰis the main component.UV results show that the polysaccharide from Lepista Sordida Sing does not contain nucleic acids and proteins.HPLC results showed that there was a significant absorption peak of polysaccharide inⅠcomponent.IR results show that several typical characteristic absorption peaks of polysaccharide within 400~4000cm-1 which proved the polysaccharides is complex polysaccharides connected by a variety of glycosidic bond of sugar.HPCE results show that the polysaccharides consists of six simple sugars including glucosamine and glucuronid acid.Conclusion:The structure and properties of polysaccharides are studied by UV,HPLC,IR,HPCE chromatographic methods,which has important guiding significance for the development of new polysaccharide health foods and medicines.
作者
杨晶
Yang Jing(Heilongjiang Agricultural Vocational and Technical College,Jiamusi 154007)
出处
《黑龙江医药》
CAS
2024年第2期302-305,共4页
Heilongjiang Medicine journal
关键词
紫丁香蘑多糖
工艺优化
图谱分析
Lepista nuda polysaccharides
Process optimization
Spectra analysis
