摘要
为了解广东地区鹅源多杀性巴氏杆菌(Pm)的流行情况、药物敏感性、耐药基因的携带情况以及耐药表型与耐药基因的相关性,本实验对2019年~2022年从广东地区采集的193份疑似感染Pm病鹅的心脏血液和肝脏组织划线接种于不同的培养基分离细菌,对分离菌纯化后采用PCR扩增分离菌的KMT1基因并测序,采用多重PCR扩增分离菌的5个荚膜基因及8个脂多糖基因,分析分离菌的荚膜分型与脂多糖(LPS)分型。结果显示分离到83株鹅源Pm,其中82株为荚膜A型,1株无法通过荚膜定型;LPS分型为L1型。采用K-B纸片扩散法检测分离菌的药物敏感性;通过PCR检测分离菌7类药物的15种耐药基因,包括β-内酰胺类(bla_(CTX-M)、bla_(TEM)、bla_(OXA))、氨基糖苷类(aadA1、aph(3’)Ila)、喹诺酮类(qnrA、qnrB、qnrS)、酰胺醇类(floR)、四环素类(tetM、tetX)、大环内酯类(ermF、ereD)、磺胺类(sul1、sul3)耐药基因。采用统计学软件SPSS22中的完全随机设计两样本率的卡方检验分析分离菌的耐药表型和相应耐药基因的相关性。药敏试验结果显示,83株鹅源Pm对多种药物敏感性较高,其中对喹诺酮类的环丙沙星和氧氟沙星、氨基糖苷类的丁胺卡那和大观霉素、β-内酰胺类的头孢曲松、磺胺类的复方新诺明、大环内酯类的阿奇霉素敏感的菌株占比均在63%以上;对氨基糖苷类的链霉素和卡那霉素耐药的菌株占比高于50%,对氨基糖苷类的新霉素和四环素类的四环素耐药的菌株分别占49.40%(41/83)和46.99%(39/83)。耐药基因的检测结果显示,分离菌检出耐药基因qnrB、sul3、blaTEM、aadA1、aph(3’)Ila、ereD、ermF、tetM、floR,检出率分别为9.63%(8/94)、27.71%(23/83)、28.92%(24/83)、48.19%(40/83)、14.46%(12/83)、49.40%(41/83)、3.61%(3/83)、1.20%(1/83)、32.53%(27/83),未检出耐药基因qnrA、qnrS、sul1、tetX、bla_(CTX-M)、bla_(OXA)。相关性分析结果显示,aadA1基因与Pm对新霉素、链霉�
To investigate the prevalence,antimicrobial sensitivity,carrying of antimicrobial resistance genes,and the correlation between resistance phenotypes and resistance genes of Pasteurella multocida(Pm) from geese in Guangdong area,193heart blood and liver samples of suspected Pm-infected geese collected from Guangdong from 2019 to 2022 were streaked and inoculated into different culture mediums to isolate bacteria.After purification,the KMT1 gene of the isolates was amplified by PCR and sequenced.The five capsular genes and eight lipopolysaccharide genes of the isolates were amplified by multiplex PCR to analyze the capsular typing and lipopolysaccharide typing of the isolates.The results showed that 83 strains of goose-derived Pm were isolated,of which 82 were of capsule type A,and one could not be classified by capsular typing;the lipopolysaccharide types were all L1.The antimicrobial sensitivity of the isolates was tested using the K-B disc diffusion method.Fifteen antimicrobial resistance genes of seven classes were detected by PCR,including β-lactams(bla_(CTX-M),bla_(TEM),bla_(OXA)),aminoglycosides(aadA1,aph(3')Ila),quinolones(qnrA,qnrB,qnrS),florfenicol(floR),tetracyclines(tetM,tetX),macrolides(ermF,ereD),and sulfonamides(sul1,sul3).The correlation between resistance phenotypes and corresponding resistance genes was analyzed using the chi-square test for two-sample rates in a completely randomized design in SPSS22.The results of antimicrobial sensitivity test showed that the83 strains of goose-derived Pm were highly sensitive to various drugs,with more than 63% of the strains being sensitive to quinolones ciprofloxacin and ofloxacin,aminoglycosides gentamicin and amikacin,β-lactams ceftriaxone,sulfonamides compound sulfamethoxazole,and macrolides azithromycin.More than 50% of the strains were resistant to aminoglycosides streptomycin and kanamycin,and 49.40%(41/83) and 46.99%(39/83) of the strains were resistant to aminoglycosides neomycin and tetracyclines tetracycline,respectively.The detection of resistan
作者
苏文楠
杨智灿
张美琳
钟嘉诚
陈亦杏
陈济铛
张溢珊
张济培
SU Wen-nan;YANG Zhi-can;ZHANG Mei-lin;ZHONG Jia-cheng;CHEN Yi-xing;CHEN Ji-dang;ZHANG Yi-shan;ZHANG Ji-pei(Foshan University of Science and Technology,Foshan 528225,China;Foshan Tianmu Biotechnology Co.,Ltd.,Foshan 528225,China)
出处
《中国预防兽医学报》
CAS
CSCD
北大核心
2024年第2期121-127,共7页
Chinese Journal of Preventive Veterinary Medicine
基金
广东省普通高校科研项目重点领域专项(2020ZDZX1018)
佛山天牧生物科技有限公司项目(BKH209059)。
关键词
多杀性巴氏杆菌
药物敏感性试验
耐药基因
Pasteurella multocida
antimicrobial sensitivity test
antimicrobial resistance gene
