摘要
为了解禽源多杀性巴氏杆菌的药物敏感性及氟苯尼考的耐药基因的分布特征,采用琼脂二倍稀释法,对分离自福建、广东和江西等南方数省部分地区113株禽源多杀性巴氏杆菌进行9种抗生素的耐药性分析,并应用PCR方法检测氟苯尼考耐药基因floR的分布情况。结果显示:上述菌株对四环素表现高水平耐药,耐药率为65.49%(74/113);对链霉素、甲氧苄啶/磺胺甲噁唑、氟苯尼考、卡那霉素与恩诺沙星表现中等水平耐药,耐药率分别是43.36%(49/113)、39.82%(45/113)、27.43%(31/113)、25.66%(29/113)与24.78%(28/113);对氨苄西林、头孢噻呋与头孢克洛较为敏感,耐药率分别为4.42%(5/113),2.65%(3/113)与1.77%(2/113);其中52.21%(59/113)菌株能耐受3类及3类以上的抗菌药物;氟苯尼考耐药基因floR的检出率为64.04%(72/113),且所有氟苯尼考耐药菌株均含有floR基因。结论:113株禽源多杀性巴氏杆菌存在多重耐药现象,且floR耐药基因较为流行。本研究结果为上述地区针对禽源多杀性巴氏杆菌临床用药提供科学依据。
In order to understand the antimicrobial sensitivity and distribution of the floR genes of Pasteurella multocida strains isolated from poultry in Fujian,Guangdong,Jiangxi and other provinces in the south of China,113 Pasteurella multocida strains were tested for antimicrobial susceptibility to 9 antibiotics by the agar dilution method.Then,the PCR method was used to detect floR.The results were as follows:The drug sensitivity test results showed that the above strains had high resistance to Tetracycline,at the rate of 65.49%(74/113);intermediate resistance to Streptomycin,Trimethoprim-sulfamethoxazole,Kanamycin,Florfenicol,and Enrofloxacin,at the rates of 43.36%(49/113),39.82%(45/113),25.66%(29/113),27.43%(31/113)and 24.78%(28/113),respectively;and low resistance to Ampicillin,Ceftiofur,Cefaclor,at the rates of 4.42%(5/113),2.65%(3/113)and 1.77%(2/113),and 59 of 113(52.21%),respectively.The Pasteurella multocida strains were of multiple resistances.The detected rates of the resistant floR was 64.04%(72/113),and all the isolates that showed resistance to Florfenicol were positive for the floR gene.The present study indicated that the Pasteurella multocida strains had a multiple drug resistance,and the prevalence of floR was very popular.These data might serve as reference for clinical research.
作者
陈红梅
程龙飞
邓辉
刘荣昌
傅光华
施少华
万春和
傅秋玲
郑庆礼
白丁平
黄小红
黄瑜
CHEN Hongmei;CHENG Longfei;DENG Hui;LIU Rongchang;FU Guanghua;SHI Shaohua;WAN Chunhe;FU Qiuling;ZHENG Qingli;BAI Dingping;HUANG Xiaohong;HUANG Yu(Fujian Animal Diseases Control Technology Center,Fujian Province Key Laboratory for Avian Diseases Control and Prevention,Institute of Animal Husbandry and Veterinary Medicine,Fujian Academy of Agricultural Sciences,Fuzhou350013,China;University Key Lab for Integrated Traditional Chinese and Western Veterinary Medicine and Animal Healthcare of Fujian Province,Fujian Agriculture and Forestry University,Fuzhou350002,China)
出处
《畜牧与兽医》
北大核心
2019年第3期93-96,共4页
Animal Husbandry & Veterinary Medicine
基金
现代农业产业技术体系建设专项资金(CARS-42-18)
福建省属公益类科研院所基本科研项目(2016R1022-2)
福建省农业科学院畜牧兽医创新团队(STIT2017-1-5)
福建省农业科学院自由探索创新项目(AA2018-19)
福建省教育厅实验室开放基金项目
