摘要
目的本研究旨在探究甘草附子汤对由尿酸钠晶体引发的大鼠痛风性关节炎的影响,并进一步研究其通过调节巨噬细胞极化途径的潜在机制。方法本实验共纳入40只SD大鼠,随机分配成对照组、模型组、秋水仙碱组和甘草附子汤组。利用MSU注射入大鼠的踝关节腔内,构建痛风性关节炎的动物模型。通过大鼠足部肿胀情况及其步态变化来评估症状严重程度;使用HE染色技术对关节组织样本进行组织学评分;通过酶联免疫吸附试验来测定大鼠血浆IL-1β、IL-10含量;采用Real-Time PCR法测定大鼠关节中iNOS、Arg-1的表达;采用免疫荧光测定TNF-α在滑膜组织的表达。结果甘草附子汤能明显减轻痛风性关节炎大鼠足跖肿胀,改善步态及组织学评分(P<0.05)。大鼠经过甘草附子汤的治疗,血清中IL-1β表达水平显著降低(P<0.05),相反IL-10表达水平显著升高,其中P<0.05;Real-Time PCR结果提示,甘草附子汤能显著降低iNOS的表达(P<0.01),增加Arg-1表达(P<0.01)。同时免疫荧光检测发现甘草附子汤抑制TNF-α的表达。结论甘草附子汤能有效抑制痛风性关节炎的发展,其作用机制可能与促进巨噬细胞从M1型向M2型的转变密切相关。
Objective To investigate the effect of Gancao Fuzi Decoction on gouty arthritis induced by sodium urate crystals in rats,and to further investigate the potential mechanism of Gancao Fuzi Decoction by regulating the polarization pathway of macrophages.Methods A total of 40 SD rats were included in this experiment and randomly assigned into control group,model group,colchicine group and Gancao Fuzi Decoction group.MSU was injected into the ankle cavity of rats to construct an animal model of gouty arthritis.The severity of symptoms was assessed by foot swelling and gait changes in rats;HE staining technique was used to score the joint tissue samples;plasma IL-1βand IL-10 were determined by enzyme-linked immunosorbent assay(ELISA);the expression of iNOS and Arg-1 in the joints of rats was determined by Real-Time PCR;the expression of TNF-αin synovial tissue was determined by immunofluorescence.Results Gancao Fuzi Decoction can significantly reduce the swelling of foot and plantar of rats with gouty arthritis,and improve gait and histological score(P<0.05).After treatment with Gancao Fuzi Decoction,serum IL-1βexpression level was significantly decreased(P<0.05),the expression level of IL-10,however,was significantly increased(P<0.05);at the same time,Real-Time PCR results showed that the expression of iNOS was significantly decreased(P<0.01)and the expression of Arg-1 was increased(P<0.01);meanwhile,immunofluorescence detection showed that Gancao Decoction inhibited the expression of TNF-α.Conclusion Gancao Fuzi Decoction demonstrates a pronounced suppressive impact on gouty arthritis.This effect is likely associated with its role in facilitating the transition of macrophages from the M1 to the M2 phenotype.
作者
戴震
韩子茹
裴俊泽
陆慧
王健
Dai Zhen;Han Ziru;Pei Junze;Lu Hui;Wang Jian(The First Affiliated Hospital of Jinzhou Medical University,Jinzhou 121000 China)
出处
《锦州医科大学学报》
CAS
2024年第2期18-23,共6页
Journal of Jinzhou Medical University
基金
辽宁省科技厅援疆项目,项目编号:2022-M S-21。
关键词
甘草附子汤
巨噬细胞极化
痛风性关节炎
Gancao Fuzi Decoction
macrophage polarization
gouty arthritis
