摘要
用Lineweaver-Burk双倒数法研究姜酚对α-淀粉酶和α-葡萄糖苷酶的动力学抑制类型。结果表明,姜酚对α-淀粉酶和α-葡萄糖苷酶均有明显的抑制效果,抑制类型分别为竞争型抑制和混合型抑制,其抑制常数K_(ic1)、K_(ic2)分别为3.88和2.67。与α-淀粉酶相比,姜酚与α-葡萄糖苷酶结合的亲和力更好。
The Lineweaver-Burk double reciprocal method was used to study the kinetic inhibition type of gingerol onα-amylase andα-glucosidase,and its interaction force was analyzed through thermodynamic parameters.The results showed that gingerol had obvious inhibitory effects onα-amylase andα-glucosidase.The inhibition types were competitive inhibition and mixed inhibition,respectively.The inhibition constants K_(ic1) and K_(ic2) were 3.88 and 2.67,respectively.Compared withα-amylase,the binding affinity of gingerol toα-glucosidase was better.
作者
徐菁
孙浩强
张校志
XU Jing;SUN Haoqiang;ZHANG Xiaozhi(Guiyang University of Information Technology,Guiyang 550025,China;Sports College of Guiyang University,Guiyang 550005,China;Guizhou Business College,Guiyang 550014,China)
出处
《现代食品》
2024年第5期201-204,共4页
Modern Food
