摘要
问荆(Equisetum arvense)是在中国广泛分布并产生严重威胁的农田杂草。本研究收集了问荆的根茎水浸液,采用生物测定法研究不同浓度问荆根茎水浸液对油菜(Brassica napus)和青菜(B.chinensis)的化感效应,为其合理生产提供理论依据。结果表明,问荆水浸液对油菜和青菜种子发芽和幼苗生长的化感作用主要表现为抑制作用,出现“根短芽长”的现象;并通过调节体内可溶性蛋白和可溶性糖含量以提高对问荆水浸液的抗抑制能力;油菜和青菜的丙二醛含量在问荆水浸液处理后显著升高,超氧化物歧化酶活性变化不显著,过氧化氢酶和过氧化物酶活性以及叶绿素含量随着水浸液浓度升高呈不同变化。研究结果表明,油菜和青菜的幼苗对问荆水浸液化感作用的响应不同。
Equisetum arvense is a widely distributed farmland weed that poses a serious threat to crops and vegetables in China.We collected aqueous extract from the roots and stems of E.arvense and used a bioassay to study the allelopathic effects of different concentrations of E.arvense aqueous extract on Brassica napus and B.chinensis,to provide a theoretical basis for their rational synthesis.Our results indicate that the allelopathic effect of E.arvense aqueous extract on B.napus and B.chinensis seed germination and seedling growth is mainly inhibitory,resulting in short root,1ong shoot of seedlings.By regulating soluble protein and soluble sugar content in vivo,B.napus and B.chinensis could improve their resistance to the stress of aqueous E.arvense extract.After treatment with aqueous extract,and with increasing aqueous E.arvense extract concentration,B.napus and B.chinensis malondialdehyde content increased significantly,but superoxide dismutase activity did not change significantly,while catalase and peroxidase activities as well as chlorophyll content showed different changes.The results showed that the allelopathic responses of B.napus and B.chinensis seedlings to aqueous E.arvense extract varied.
作者
何智荣
路曼晴
刘慧君
王源秀
HE Zhirong;LU Manqing;LIU Huijun;WANG Yuanxiu(College of Life Sciences,Anhui Normal University,Wuhu 241000,Anhui,China)
出处
《草业科学》
CAS
CSCD
北大核心
2024年第4期899-907,共9页
Pratacultural Science
基金
安徽省高校科学研究重大项目(NO.KJ2020ZD07)
安徽省大学生创新创业训练计划项目(202110370095)。
关键词
问荆水浸液
油菜
青菜
化感效应
发芽趋势
生物量
生理活性
aqueous Equisetum arvense extract
Brassica napus
Brassica chinensis
allelopathic effects
germination trend
biomass
physiological activity
