摘要
目的研究胰岛素样生长因子ⅡmRNA结合蛋白3(IMP3)与宫颈癌患者肿瘤微环境中免疫CD8^(+)T细胞浸润的相关性。方法选取2019年6月—2020年6月于杭州市临平区第一人民医院就诊的宫颈癌患者80例,使用免疫组化检测癌组织与癌旁组织IMP3阳性表达率、免疫CD8^(+)T细胞浸润数量,分析宫颈癌中IMP3阳性表达、免疫CD8^(+)T细胞浸润数量及与患者临床特征的关系,宫颈癌IMP3表达与肿瘤微环境免疫CD8^(+)T细胞浸润数量的相关性,比较IMP3阳性/阴性表达、CD8^(+)T细胞浸润低/高浸润与患者3年生存率的关系。结果与癌旁组织比较,癌组织IMP3阳性表达率(81.25%vs.12.50%)升高,差异有统计学意义(χ^(2)=75.922,P<0.05)。与癌旁组织比较,癌组织CD8^(+)T细胞浸润数量[(13.32±2.09)个/HP vs.(37.86±4.31)个/HP]减少,差异有统计学意义(t=45.823,P<0.05)。TNM分期Ⅲ~Ⅳ期(92.50%vs.70.00%)、有淋巴结转移(93.94%vs.72.34%)、深肌层浸润(94.59%vs.69.77%)及低分化(95.83%vs.75.00%)患者IMP3阳性表达率较高,差异均有统计学意义(χ^(2)=6.646、5.937、8.047、4.786,均P<0.05)。TNM分期Ⅲ~Ⅳ期[(10.98±1.54)个/HP vs.(16.75±3.52)个/HP]、有淋巴结转移[(9.89±1.07)个/HP vs.(15.64±2.41)个/HP]、深肌层浸润[(11.32±1.24)个/HP vs.(14.35±2.29)个/HP]及分化程度越低[(9.78±1.12)个/HP vs.(16.47±2.48)个/HP]患者CD8^(+)T细胞浸润数量越少,差异均有统计学意义(t=9.498、13.792、7.188、12.639,均P<0.05)。IMP3阳性表达的宫颈癌组织中有少量CD8^(+)T细胞浸润,IMP3阴性表达的宫颈癌组织中有大量CD8^(+)T细胞浸润,IMP3表达与CD8^(+)T细胞浸润数量呈负相关(r=-0.613,P<0.05)。IMP3阳性表达患者3年生存率(58.46%)显著低于IMP3阴性表达患者(93.33%),差异有统计学意义(χ^(2)=6.515,P<0.05)。CD8^(+)T细胞低浸润患者3年生存率(56.67%)低于CD8^(+)T细胞高浸润患者(90.00%),差异有统计学意义(χ^(2)=7.326,P<0.05)。结论IMP3在宫颈癌中表达升高,I
Objective To study the relationship between Insulin-like growth factor Ⅱ mRNA binding protein 3(IMP3)and the infiltration of immune CD8^(+)T cells in the Tumor microenvironment of cervical cancer patients.Methods 80 cases of cervical cancer patients in our hospital from June 2019 to June 2020 were selected.Immunohistochemistry was used to detect the positive expression rate of IMP3 and the number of immune CD8^(+)T cells infiltrated in cancer tissues and adjacent tissues.The positive expression of IMP3,the number of immune CD8^(+)T cells infiltrated in cervical cancer and their relationship with patients'clinical characteristics were analyzed.The correlation between the expression of IMP3 in cervical cancer and the number of immune CD8^(+)T cells infiltrated in the Tumor microenvironment was compared The relationship between low/high infiltration of CD8^(+)T cells and 3-year survival rate of patients.Results Compared with adjacent tissues,the positive expression rate of IMP3 in cancer tissue(81.25%vs.12.50%)increased(χ^(2)=75.922,P<0.05).Compared with adjacent tissues,the number of CD8^(+)T cell infiltration in cancer tissue decreased(t=45.823,P<0.05).Patients with TNM stages Ⅲ-Ⅳ(92.50%vs.70.00%),lymph node metastasis(93.94%vs.72.34%),deep muscle infiltration(94.59%vs.69.77%),and poorly differentiated(95.83%vs.75.00%)have a higher positive expression rate of IMP3(χ^(2)=6.646,5.937,8.047,4.786,P<0.05).TNM staging stages Ⅲ-Ⅳ[(10.98±1.54)pieces/HP vs.(16.75±3.52)pieces/HP],with lymph node metastasis[(9.89±1.07)pieces/HP vs.(15.64±2.41)pieces/HP],deep muscle infiltration[(11.32±1.24)pieces/HP vs.(14.35±2.29)pieces/HP],and lower differentiation[(9.78±1.12)pieces/HP vs.(16.47±2.48)pieces/HP],patients with less CD8^(+)T cell infiltration(t=9.498,13.792,7.188,12.639,P<0.05).There is a small amount of CD8^(+)T cell infiltration in cervical cancer tissues with positive expression of IMP3,while a large number of CD8^(+)T cell infiltration is present in cervical cancer tissues with negative expression of
作者
谌伽莉
练慧
黄炎
CHEN Jia-li;LIAN Hui;HUANG Yan(Department of Pathology,The First People's Hospital of Linping District,Hangzhou,Zhejiang 311100,China)
出处
《中国妇幼保健》
CAS
2024年第8期1505-1509,共5页
Maternal and Child Health Care of China
基金
浙江省医学会临床科研基金项目(2019ZYC-A99,2019ZYC-A101)。
