摘要
以威氏绿绒蒿(Meconopsis wilsonii)花蕾期、开裂期、全展期的花瓣为材料,采用紫外分光光度法、火焰原子吸收法对不同时期花瓣中类黄酮含量和金属离子含量进行测定,以期发现类黄酮、金属离子与威氏绿绒蒿花瓣呈色之间的关系。结果表明,威氏绿绒蒿花瓣类黄酮含量随花瓣发育的总体变化规律为前期增加、后期减少,类黄酮含量在各个时期存在显著性差异(P<0.05)。金属元素含量以K、Ca、Mg居多,Cu元素含量始终维持在最低水平;其中Fe、Ca、K 3种元素与花色显色呈正相关,含量变化整体呈先下降后上升趋势,同时类黄酮含量变化与此3种元素含量变化一定程度上存在相似性,在花蕾期白色阶段(B1时期)3种元素含量较其他时期都高,随类黄酮含量积累至花蕾期粉紫色阶段(B2时期)3种元素含量减少,推测Fe、Ca、K 3种元素与类黄酮结合形成复合物;Zn元素在各时期变化并不明显,推测Zn元素一定程度上参与花色形成,并不是主要参与者。本研究通过对威氏绿绒蒿不同时期花瓣中类黄酮含量和金属离子含量进行测定,进一步深化对其花色形成的认知,以期为绿绒蒿花色研究提供理论依据。
The petals of Meconopsis wilsonii at bud stage,cracking stage and full extension stage were used as materials,the contents of flavonoids and metal ions in the petals of Meconopsis wilsonii at different stages were determined by ultraviolet spectrophotometry and flame atomic absorption spectrometry,in order to find the relationship between flavonoids,metal ions and petal coloration of Meconopsis wilsonii.The results showed that the general change rule of flavonoid content with petal development was increased in the early stage and decreased in the later stage,and there were significant differences in flavonoid content in each stages(P<0.05).The content of metal elements K,Ca and Mg was the most,and the content of Cu was always maintained at the lowest level.The contents of Fe,Ca and K were positively correlated with the color development of flower and color,and the changes of the contents of Fe,Ca and K were firstly decreased and then increased.At the same time,the changes of the contents of flavonoids were similar to the changes of the contents of these three elements to a certain extent.The contents of the three elements were higher in the white stage of bud stage(B1 stage)than in other stages.With the accumulation of flavonoids to the pink purple stage(B2 stage),the contents of the three elements decreased,and it was speculated that Fe,Ca,K elements combined with flavonoids to form complex.The change of Zn element is not obvious in each period,so it is speculated that Zn element is involved in the formation of flower color to some extent,but is not the main participant.The contents of flavonoids and metal ions in the petals of Meconopsis wilsonii in different periods were determined to further deepen the cognition of the flower color formation,in order to provide a theoretical basis for the study of the flower color of Meconopsis vermosi.
作者
张冰
郭小雪
罗军
区智
屈燕
Zhang Bing;Guo Xiaoxue;Luo Jun;Ou Zhi;Qu Yan(Yunnan Engineering Research Center for Functional Flower Resources and Industrialization,Southwest Research Center for Engineering Technology of Landscape Architecture(State Forestry and Grassland Administration),College of Landscape Architecture and Horticulture Sciences,Southwest Forestry University,Kunming,650224)
出处
《分子植物育种》
CAS
北大核心
2024年第9期3103-3110,共8页
Molecular Plant Breeding
基金
国家自然科学基金项目(32160404,31460218)
云南省万人计划青年拔尖人才项目(YNWR-QNBJ-2019-211)共同资助。
关键词
威氏绿绒蒿
花色
类黄酮
金属元素
Meconopsis wilsonii
Flower color
Flavonoids
Metal element
