摘要
VQ家族是一类植物特有的家族,被报道参与生长发育、生物和非生物逆境胁迫响应等过程。本研究利用生物信息学和比较基因组学的方法,在烟草基因组中对VQ家族成员进行鉴定,并进行进化分析、共线性分析、复制分析以及表达模式等分析。本研究从烟草基因组中鉴定出57个VQ家族成员编码基因,其中33个普通烟草VQ基因被锚定到染色体上。系统发育分析发现,普通烟草和拟南芥的VQ家族成员被分成了9个亚家族。共线性分析表明,普通烟草NtVQ23与拟南芥中的AtVQ16形成共线性基因对。同时,对烟草基因组中的复制事件分析表明,全基因组重复事件在VQ家族成员的扩张中发挥着重要作用。此外,还对部分VQ家族成员的表达模式进行了分析,结果表明VQ基因的表达具有组织特异性,NtVQ9、NtVQ26等基因能够被青枯病接种和盐胁迫处理显著诱导。本研究对普通烟草VQ家族成员进行了鉴定与分析,为其后续研究VQ家族成员的功能提供了一定基础。
The VQ family represent a plant specific family and play multiple roles in development,biotic and abiotic stress responses.In this study,bioinformatics and comparative genomics methods were used to identify VQ family members in tobacco genome,and evolutionary analysis,collinearity analysis,replication analysis and expression pattern analysis were performed.In current study,a total of 57 VQ family members were identified in tobacco genome,while 33 of those were anchored on chromosomes successfully.The phylogenetic analysis revealed that the newly identified tobacco VQ family members together with reported Arabidopsis members fall into 9 subfamilies.The collinearity analysis showed that NtVQ23 in tobacco formed collinearity gene pairs with AtVQ16 in Arabidopsis.Meanwhile,analysis of replication events in the tobacco genome shows that genome-wide repeat events play an important role in the expansion of VQ family members.In addition,the expression patterns of some VQ family members were also analyzed.The results showed that the expression of VQ gene was tissue-specific,and NtVQ9,NtVQ26 and other genes could be significantly induced by bacterial wilt inoculation and salt stress treatment.In this study,VQ family members of tobacco were identified and analyzed,which provided a basis for further study on the function of VQ family members.
作者
王东
李志远
周平
蒲文宣
毛辉
王雪云
张新要
刘万峰
陈前锋
李晓旭
Wang Dong;Li Zhiyuan;Zhou Ping;Pu Wenxuan;Mao Hui;Wang Xueyun;Zhang Xinyao;LiuWanfeng;Chen Qianfeng;Li Xiaoxu(Technology Center,China Tobacco Hunan Industrial Co.,Ltd.,Changsha,410007;Key Laboratory of Tobacco Gene Resources,Tobacco Research Institute,Chinese Academy of Agricultural Sciences,Qingdao,26610l;Technology Center,China Tobacco Hubei Industrial Co.,Ltd.,Wuhan,430017;Xiangxi Branch of Hunan Provincial Tobacco Company,Jishou,416000)
出处
《分子植物育种》
CAS
北大核心
2024年第9期2856-2863,共8页
Molecular Plant Breeding
基金
中国烟草总公司科技重大专项[110201801030(JY-07)]
湖南中烟工业有限责任公司面上项目(KY2019YC-0007)
湖南中烟工业有限责任公司面上项目(KY2019YC0003)共同资助。
