摘要
目的探究水飞蓟提取物(SME)及其复合制剂对乙醇性肝损伤的保护作用。方法通过灌胃12mL·kg~150%乙醇建立乙醇肝损伤模型。将小鼠随机分为空白组(给予蒸馏水)、模型组(乙醇肝损伤模型)、SME低和高剂量组(分别给予0.06、0.20g·kg^(-1)SME)、SME+灵芝提取物(GLE)低和高剂量组(分别给予0.10、0.30g·kg^(-1)SME+GLE,SME:GLE=1:1)、水飞蓟丹参片(JAS)低和高剂量组(分别给予0.68、2.04g·kg^(-1)JAS),每组12只。用全自动生化分析仪测定法测定小鼠血清中谷丙转氨酶(GPT),用酶联免疫吸附试验法测定小鼠血清中白细胞介素-6(IL-6)和肿瘤坏死因子-α(TNF-α)的水平,用紫外分光光度计测定肝组织氧化应激指标[过氧化氢酶(CAT)和总超氧化物歧化酶(T-SOD)]。结果空白组、模型组、SME低和高剂量组、SME+GLE低和高剂量组、JAS低和高剂量组的T-SOD含量分别为(192.54±49.00)、(141.65±34.72)、(205.83±32.77)、(191.68±25.83)、(192.31±28.79)、(177.82±32.61)、(218.58±74.80)和(210.24±31.65)U·mg·prot,CAT含量分别为(37.78±5.73)、(28.92±8.44)、(44.12±11.52)、(41.41±9.15)、(47.01±10.48)、(41.63±8.95)、(47.14±8.91)和(48.29±10.06)U·mg^(-1),GPT含量分别为(47.61±13.00)、(97.84±26.00)、(62.33±18.92)、(51.84±17.91)、(70.77±28.00)、(58.00±21.27)、(52.28±18.78)和(45.55±9.27)U·L^(-1),IL-6水平分别为(21.03±1.52)、(28.43±5.75)、(21.90±3.24)、(21.23±1.55)、(22.26±2.58)、(21.24±2.91)、(22.17±4.14)和(21.14±3.02)pg·mL^(-1);模型组的上述指标与空白组比较,SME组、SME+GLE组和JAS低、高剂量组的上述指标与模型组比较,在统计学上差异均有统计学意义(均P<0.01)。空白组、模型组、SME低和高剂量组、SME+GLE低和高剂量组、JAS低和高剂量组的TNF-α水平分别为(28.07±7.72)、(69.02±16.34)、(40.29±8.94)、(48.84±10.17)、(41.91±14.96)、(40.07±12.75)、(50.72±11.44)和(45.05±11.34)pg·mL^(-1)。模型组与空白组比较,SME低和高剂�
Objective To investigate the protective effect of silymarin extract(SME)and its complex preparation on ethanol liver injury.Methods An ethanol liver injury model was established by gavage of 12 mL·kg^(-1)50% ethanol.Male mice were divided into blank group(distilled water),model group(ethanol liver injury model),SME-L,-H groups(6,20 mg·mL^(-1)SME),SME+Ganoderma lucidum extract(CLE)-L,-H groups(10,30 mg·mL^(-1)SME+CLE,SME:CLE=1:1),Jian An Shi Silymarin Pueraria Mirifica and Tansy tablets(JAS)-L,-H groups(68,204 mg·mL^(-1)JAS),there were 12 mice in each group.The serum levels of glutamic-oxaloacetic transaminase(GOT)in mice were measured by fully automated biochemical analyzer assay;the serum levels of interleukin-6(IL-6)and tumor necrosis factor-alpha(TNF-α)in mice were measured by enzyme-linked immunosorbent assay(ELASA);the hepatic tissue of oxidative stress indexes[catalase(CAT)and total superoxide dismutase(T-SOD)]were measured by ultraviolet spectrophotometer.Results The T-SOD activity in the blank group,model group,SME-L,SME-H,SME+GLE-L,SME+CLE-H,JAS-L and JAS-H groups were(192.54±49.00),(141.65±34.72),(205.83±32.77),(191.68±25.83),(192.31±28.79),(177.82±32.61),(218.58±74.80)and(210.24±31.65)U·mg·prot^(-1);CAT activity were(37.78±5.73),(28.92±8.44),(44.12±11.52),(41.41±9.15),(47.01±10.48),(41.63±8.95),(47.14±8.91)and(48.29±10.06)U·mg^(-1);CPT levels were(47.61±13.00),(97.84±26.00),(62.33±18.92),(51.84±17.91),(70.77±28.00),(58.00±21.27),(52.28±18.78)and(45.55±9.27)U·^(-1);IL-6 levels were(21.03±1.52)),(28.43±5.75),(21.90±3.24),(21.23±1.55),(22.26±2.58)(21.24±2.91),(22.17±4.14)and(21.14±3.02)pg·mL^(-1).Comparing the above indexes in the model group with the blank group,and comparing the above indexes in the SME-L,SME-H,SME+GLE-L,SME+GLE-H,JAS-L,JAS-H groups with the model group,the differences were statistically significant(all P<0.O1).The TNF-αlevels in blank,model,SME-L,SME-H,SME+CLE-L,SME+GLE-H,JAS-L and JAS-H groups werewere(28.07±7.72),(69.02±16.34),(40.29±8.94
作者
王亭乔
任吴疆
吕梁彧
魏嵘
郭月逸
赵建
WANG Ting-qiao;REN Wu-jiang;LU Liang-yu;WEI Rong;GUO Yue-yi;ZHAO Jian(School of Biological Chemical Engineering,Bejing Union University,Beijing 100023,China;Beijing Key Laboratory Of Bioactive Substances and Functional Foods,Bejing Union University,Beijing 100023,China;Chemical Engineering and Bioengineering,Donghua University,Shanghai 201620,China)
出处
《中国临床药理学杂志》
CAS
CSCD
北大核心
2024年第7期1009-1013,共5页
The Chinese Journal of Clinical Pharmacology
关键词
水飞蓟提取物
护肝
抗氧化能力
炎性因子
thistle extract
protect the liver
antioxidant capacity
inflammatory factors
