摘要
以‘艳椒465’和‘魔鬼椒’为材料,克隆获得2个辣椒(Capsicum annuum)品种酰基转移酶3(acyltransferase 3,AT3)基因序列,采用生物信息学方法预测AT3的理化性质和分子结构,进行系统进化树的构建,测定花后30 d不同组织中AT3基因的相对表达量和果实中辣椒素含量,探究不同品种AT3基因的序列差异、基因组织表达模式及其在辣椒素合成中的调控作用。结果表明克隆得到‘艳椒465’和‘魔鬼椒’AT3基因的c DNA序列,全长分别为1310和1313 bp,分别编码396和397个氨基酸,均为稳定亲水的非跨膜蛋白,并且无信号肽,是非分泌蛋白,通过转拟南芥原生质体初步定位于细胞质和细胞核中,二级结构主要是无规则卷曲和α-螺旋,磷酸化位点存在一定差异。在辣椒植株果实中的AT3基因表达量显著高于叶片和茎段,并且‘魔鬼椒’果实中的AT3基因表达量高于‘艳椒465’,‘魔鬼椒’果实中的辣椒素和二氢辣椒素含量显著高于‘艳椒465’。不同辣椒品种AT3基因序列高度相似,但其在植株不同器官的表达量及辣椒素类合成积累量有较大差异,为进一步探知辣椒素类合成通路效率差异提供一定参考。
Using 'Yan Jiao 465' and 'Devil Jiao' as materials,the gene sequences of acyltransferase 3(AT3) in two pepper varieties(Capsicum annuum) were cloned.Using bioinformatics methods to predict the physicochemical properties and molecular structure of AT3,a phylogenetic tree was constructed.The relative expression of AT3 gene in different tissues and the capsaicin content in fruits were measured 30 days after anthesis.To explore the sequence differences,tissue expression patterns,and regulatory roles of AT3 genes in capsaicin synthesis among different varieties.The results showed that the c DNA sequences of the AT3 gene of 'Yan Jiao 465' and 'Devil Jiao' were cloned,with a total length of 1 310 and 1 313 bp,respectively.They encode 396 and 397 amino acids,respectively.AT3 is a non transmembrane protein,which has stability and hydrophilicity.And AT3 is a non secretory protein with non signal peptide.The protoplasts of Arabidopsis thaliana were initially localized in the cytoplasm and nucleus.The secondary structure of AT3 protein is mainly randon coil and α-helix.There are certain differences in phosphorylation sites.The expression of AT3 gene in fruits of pepper plants was significantly higher than those in leaves and stems.The relative expression of AT3 gene in the fruit of 'Devil Pepper' were higher than those of 'Yan Jiao 465'.The contents of capsaicin and dihydrocapsaicin in the fruit of 'Devil Pepper' were significantly higher than those of 'Yan Jiao 465'.The AT3 gene sequences of different pepper varieties are highly similar,but there are significant differences in their expression and capsaicin synthesis accumulation in different organs of the plant.These results provide some reference for further exploring the efficiency differences of capsaicin synthesis pathways.
作者
吴媛
苏世贤
常媚瑕
汪淘
龙茶
韦金江
江苏燕
蓬桂华
何磊
袁远国
李伟
WU Yuan;SU Shixian;CHANG Meixia;WANG Tao;LONG Cha;WEI Jingjiang;JIANG Suyan;PENG Guihua;HE Lei;YUAN Yuanguo;LI Wei(College of Agriculture,Guizhou University,Guiyang 550025,China;Industry Technology Research Academy of Pepper,Guizhou University,Guiyang 550025,China;Research Institute of Pepper,Guizhou Province,Zunyi,Guizhou 563000,China;Guizhou Horticulture Research Institute,Guiyang 560006,China;Engineering Research Center for Protected Vegetable Crops in Higher Learning Institutions of Guizhou Province,Guiyang 550025,China)
出处
《植物生理学报》
CAS
CSCD
北大核心
2024年第2期377-388,共12页
Plant Physiology Journal
基金
贵州省科技支撑计划定向重点项目(黔科合支撑[2022]重点010号)
贵州省高等学校设施蔬菜工程研究中心平台建设项目(黔教技[2022]040号)
贵州省科技成果应用及产业化计划重点项目(黔科合成果[2020]1Z005号)
国家自然科学基金(31760576)
贵州省农业科技支撑项目(黔科合支撑[2016]2548号)
农业农村部/贵州省农业农村厅贵州省朝天椒优势产业集群建设项目(农产发[2020]2号和黔农发[2020]67号)。
关键词
辣椒
AT3基因
克隆
生物信息学分析
亚细胞定位
表达差异
pepper(Capsicum annuum)
AT3 gene
clone
bioinformatics analysis
subcellular localization
expression differences