摘要
【目的】在哺乳动物中,Toll样受体和抗菌肽基因是先天免疫系统的重要组成部分,在对抗病原体侵袭过程中起至关重要的作用。针对藏猪和杜长大三元猪的Toll样受体和抗菌肽基因在不同免疫器官或组织中的表达差异进行探索,以揭示这些基因对于抗病能力和免疫反应的潜在贡献,为筛选抗病分子标记提供理论支撑。【方法】通过qPCR对6月龄藏猪和杜长大三元猪肺脏、肠系膜淋巴结、腹股沟淋巴结、颌下淋巴结和脾脏中Toll样受体基因(TLR1~TLR9)和两种抗菌肽基因(PBD-1和PR-39)的mRNA丰度进行检测。【结果】在藏猪的免疫器官或组织中Toll样受体与抗菌肽基因的m RNA表达大多数显著高于杜长大三元猪。其中,肺脏中TLR1、TLR2的m RNA表达量均提高50%左右,PR-39提高2.6倍;肠系膜淋巴结中TLR4的表达提高40%,TLR1、PR-39的表达分别提高88%和3倍;腹股沟淋巴结中TLR1、TLR2的表达提高约2倍,TLR9、PR-39的表达提高70%,PR-39的表达更是提高了7倍多;颌下淋巴结中TLR1、TLR2、TLR4、TLR7的表达均高出2倍以上,PR-39的表达也高出近7倍,它与腹股沟淋巴结中的水平相近;脾脏中TLR1的表达上升3.5倍,与在颌下淋巴结中的表达结果相似。TLR4、TLR9的表达上升50%左右,PR-39的表达上升2.5倍。【结论】藏猪相对于杜长大三元猪,在多个免疫器官或组织中的Toll样受体和抗菌肽基因表现出更高的表达水平。暗示藏猪可能拥有更强大的先天免疫能力,能够产生更有效的局部或系统免疫反应对抗病原微生物的感染。研究结果为识别抗病分子标记提供了重要的理论支持,有望为进一步改良藏猪和其他猪种的抗病性提供科学依据。
【Objective】In mammals,Toll-like receptors and antimicrobial peptide genes are important components of the innate immune system,which play a crucial role in fighting against pathogen attacks.The study was conducted to explore the expression differences of Toll-like receptor and antimicrobial peptide genes between different immune organs or tissues in Tibetan pigs and Duroc-Landrace-Yorkshire pigs,with an aim to reveal the potential contribution of these genes to disease resistance and immune response and provide theoretical support for the screening of molecular markers for disease resistance.【Method】The mRNA abundance of Toll-like receptors genes(TLR1-TLR9) and two types of antimicrobial peptide genes(PBD-1 and PR-39) in lungs,mesenteric lymph nodes,inguinal lymph nodes,submandibular lymph nodes and spleens of 6-month-old Tibetan and Duroc-Landrace-Yorkshire pigs were detected by qPCR.【Result】The mRNA expression of Tolllike receptors and antimicrobial peptide genes in most of the immune organs or tissues of Tibetan pigs was significantly higher than that of Duroc-Landrace-Yorkshire pigs.Among them,the mRNA expression of TLR1 and TLR2 in lungs was increased by about 50%,and PR-39 was increased by 2.6 times;the expression of TLR4 in mesenteric lymph nodes was increased by 40%,and the expression of TLR1 and PR-39 was increased by 88% and 3 times,respectively.In the inguinal lymph nodes,the expression of TLR1 and TLR2 was increased by about 2 times,and the expression of TLR9 and PR-39 was increased by 70%,especially,the expression of PR-39 increasing by 7 times;The expression of TLR1,TLR2,TLR4 and TLR7 in submandibular lymph nodes was increased by more than 2 times,and the expression of PR-39 was increased by nearly 7 times,which was similar to that in inguinal lymph nodes;the expression of TLR1 in the spleen rose by 3.5 times,which was similar to that in submandibular lymph nodes.The expression of TLR4 and TLR9 increased by about 50%,and the expression of PR-39increased by 2.5 times.【Conclusion】Tibet
作者
李新明
洪纯
黄秋艳
程蕾燕
孟繁明
朱向星
唐冬生
王塑天
LI Xinming;HONG Chun;HUANG Qiuyan;CHENG Leiyan;MENG Fanming;ZHU Xiangxing;TANG Dongsheng;WANG Sutian(School of Life Sciences and Engineering,Foshan University/Guangdong Key Laboratory of Animal Molecular Design and Precise Breeding,Foshan 528000,China;Institute of Animal Science,Guangdong Academy of Agricultural Sciences/State Key Laboratory of Swine and Poultry Breeding Industry/Guangdong Key Laboratory of Animal Breeding and Nutrition,Guangzhou 510610,China;Medical School,Foshan University/Guangdong Research Center of Gene Editing Engineering Technology,Foshan 528000,China)
出处
《广东农业科学》
CAS
2024年第3期114-123,共10页
Guangdong Agricultural Sciences
基金
广东省基础与应用基础研究基金温氏联合基金项目(2019B1515210013)
广东省重点领域研发计划项目(2022B0202090002)
国家重点实验室建设项目(ZQQZ-38)
广东省农业科学院协同创新中心项目(XTXM202203-XT202217)。
关键词
TOLL样受体
抗菌肽
先天免疫
抗病性
qPCR
藏猪
Toll-like receptor
antimicrobial peptide
innate immunity
disease resistance
qPCR
Tibetan pig
