摘要
以“众里寻他千百度——核酸检测的应用”实验项目为例,探索将科研项目的部分内容应用于高校生物类通识课程的实践经验。该项目以拟南芥T-DNA插入突变体为材料,通过基因组DNA的提取、PCR检测和条带分析等,探讨核酸检测技术在科学研究和医学检验中的应用,涵盖一个完整的富含生物学特色的科研过程。该项目在通识课程中的实践,使非生物专业的学生在获得生物学核酸提取相关知识点和实验技能点的同时,了解生物学科研项目的实施过程;同时,通过鼓励学生思考核酸检测技术手段发展的背景、过程和意义,培养学生的发散性思维和创新实践能力,为培养新时代全面创新型人才奠定基础。
The experimental project of"searching for others-application of nucleic acid detection"was summarized here,and the discussion of practical experience of applying part of the research project to the undergraduate general education course was performed.In this experiment project,the Arabidopsis thaliana T-DNA insertion mutants were used as materials to explore the application of nucleic acid detection technology in scientific research and medical testing through genomic DNA extraction,PCR detection and band analysis,covering a complete scientific research process.The practice of this experiment enables non-biology majors to understand the implementation process of biology scientific research projects while acquiring knowledge in biology and experimental skill points related to nucleic acid extraction in biology.It encourages students to think about the background,process and significance of the development of technical means in the nucleic acid testing,cultivating dispersive thinking,innovation and practice ability of students,and laying the foundation for the cultivation of comprehensive and innovative talents in the new era.
作者
阎臻
杨健
熊莉
樊佳
张大伟
林宏辉
刘唤唤
YAN Zhen;YANG Jian;XIONG Li;FAN Jia;ZHANG Dawei;LIN Honghui;LIU Huanhuan(Virtual Simulation Experiment Teaching Center of Biological Science and Technology,National Demonstration Centerfor Experimental Biology Education,College of Life Sciences,Sichuan University,Chengdu 610064,China)
出处
《生物学杂志》
CAS
CSCD
北大核心
2024年第2期126-130,共5页
Journal of Biology
基金
国家自然科学基金面上项目(31870244)
四川大学基础学科拔尖学生培养研究课题(SCUBJ112)
四川大学高等教育教学改革工程(第十期)项目(SCU10002)。
关键词
科研项目转化
通识课程
实验教学
核酸检测
transformation of scientific research project
general education course
experimental teaching
nucleic acid test
