摘要
目的利用原位杂交联合免疫荧光建立EB病毒编码的小RNA(EBER)的荧光双重标记方法。方法收集2018年12月至2019年11月于首都医科大学附属北京友谊医院病理科诊断为淋巴瘤患者的蜡块20例,其EBER原位杂交检测结果均为阳性。每例取蜡块进行连续切片,每例切片2张,其中1张切片进行荧光双重标记,另1张作为阴性对照。通过荧光显微镜查看双重荧光标记的效果。结果荧光双重标记成功后细胞和组织结构完整。原位杂交联合免疫荧光阳性的细胞核呈绿色荧光,免疫荧光阳性的细胞膜呈红色荧光,双重标记阳性细胞的膜与核红绿荧光对比鲜明。结论建立了一种新的EBER荧光双重标记方法,且此方法可以用于判断EB病毒感染的细胞属于B细胞还是T细胞,有助于淋巴瘤的诊断。
Objective To establish the double fluorescent labeling technique of using in situ hybridization(ISH)combined with immunofluorescence(IF)for EBV-encoded RNA(EBER)detection.Methods Twenty cases diagnosed with lymphoma from December 2018 to November 2019 in Department of Pathology,Beijing Friendship Hospital,Capital Medical University were collected and presented EBV-positive by ISH.The specimen of each case was sectioned into 2 sequential slices,one was prepared for the double fluorescent labeling,the other one was prepared for negative control.The result of double fluorescent labeling was read on fluorescence microscopy.Results The structures of tissues and cells were complete and legible.The green fluorescence was observed in nucleus by ISH combined with IF and the red fluorescence was observed in cytomembrane by IF.The fluorescence distinction of nucleus and cytomembrane was obvious on double fluorescent labeling cells.Conclusion A new double fluorescent labeling technique for EBER detection is estabished.This technique could be used to identify the lineage of EBV-infected cells,such as T-cells or B-cells.This technique could possibly be a suitable method for the lymphoma diagnosis.
作者
毕阔
梅雪
周小鸽
孙岚
刘伟
BI Kuo;MEI Xue;ZHOU Xiao-ge(Department of Pathology,Beijing Friendship Hospital,Capital Medical University,Beijing 100050,China)
出处
《临床和实验医学杂志》
2020年第5期559-561,共3页
Journal of Clinical and Experimental Medicine
基金
首都卫生发展科研专项项目(编号:首发2018-1-2151).
