摘要
以吉林省水稻主推品种“吉粳809”为母本,以Bt转基因水稻材料PP-121、PP-123及PP-136为父本进行杂交,系谱法筛选获得杂交后代材料Yj-276、Yj-231及Yj-288,通过外源基因的遗传表达及抗性研究,评价杂交后代育种价值。外源基因的遗传分析表明,cry1C和bar在杂交后代中连锁遗传并共分离;酶联免疫吸附剂(ELISA)分析表明,杂交后代中cry1C蛋白表达量正常,最高可达1.78μg/g;抗性鉴定结果表明,杂交后代中二化螟抗虫性和除草剂Basta 1.0×10^(-3)mol/L耐性均>95%;农艺性状分析表明,部分杂交后代综合农艺性状优于杂交亲本“吉粳809”,此结果为水稻抗虫育种提供新种质资源及理论依据。
In this study,Bt transgenic rice lines PP-121,PP-123 and PP-136 were crossed with Jijing 809,the main rice materials in Jilin province.The hybrid lines of Yj-276,Yj-231 and Yj-288 were obtained and the breeding value was evaluated through genetic expression and resistance evaluation of foreign genes.The results of genetic analysis showed that foreign genes of crylC and bar were linked and co-segregated in the hybrid progenies.Enzyme Linked Immunosorbent Assay(ELISA)showed that cry1C protein was expressed normally in hybrid lines,up to 1.78μg/g.Insect and herbicide resistance evaluation showed that three hybrid lines were highly resistant to striped stem borers and Basta 1.0×10^(-3)mol/L compared to female parents.Agronomic character analysis showed that some hybrid progenies had better performance than that of Jijing809.This study provides new germplasm resources and a theoretical basis for insect-resistant rice breeding.
作者
宋子叶
林秀峰
严一字
金永梅
SONG Ziye;LIN Xiufeng;YAN Yizi;JIN Yongmei(College of Agricultural Sciences,Yanbian University,Yanji 133002,China;Institute of Agriculural Biotechnology,Jilin Academy of Agricultural Sciences,Changchun 130033,China;Jilin Provincial Key Laboratory of Agricultural Biotechnology,Changchun 130033,China)
出处
《吉林农业大学学报》
CAS
CSCD
北大核心
2024年第1期10-16,共7页
Journal of Jilin Agricultural University
基金
吉林省农业科技创新工程项目(CXGC2017TD009)
国家转基因重大项目(2016ZX08001001-001-007)。
关键词
抗虫转基因水稻
杂交后代
基因表达效应
抗性鉴定
insect-resistant transgenic rice
hybrid line
gene expression effect
resistance evaluation
