摘要
以密阳46(MY46)为轮回亲本,采取连续回交,结合GUS标记基因辅助选择技术,育成了Bt基因近等基因系抗螟虫密阳46(RIMY46).经考察,RIMY46及其所配杂种II32A/RIMY46的GUS反应和PCR检测均为阳性,且高抗螟虫.在早期营养生长阶段,RIMY46苗高比MY46低,分蘖数比MY46少,表明纯合态Bt基因对纯系植株的前期生长进程有一定延缓作用,这种影响随着植株长大而减小,最终除导致抽穗延迟和整精米率降低外,对其余8个农艺性状和9个稻米品质性状的影响则均不显著.比较Bt近等基因系所配杂种II32A/RIMY46和II32A/MY46有关性状的表现差异,结果则发现杂合态Bt基因对杂种F1性状表达均无明显的负作用.
With Milyang 46 (MY46) taken as recurrent line and a transgenic Bt rice (KMD1) as donor line, an isogenic line with Bt gene (RIMY46) was constructed by consecutive backcrosses and selections with GUS reaction as a marker. It was found that GUS reaction and PCR amplification test for Bt gene were positive with all RIMY46 and II32A/RIMY46 hybrid plants, and all lines were highly resistant to lepidopteran insects. In early vegetative stage, the plant height and tiller number were lower for RIMY46 than those for MY46. However, in all late stages, eight agronomic traits and nine quality traits were almost identical except for late heading date and lower head milled rice percentage in RIMY46. The crosses of II32A/RIMY46 and II32A/MY46 showed no difference in all traits investigated, indicating that Bt gene had no negative effects on the hybrid rice.
出处
《浙江大学学报（农业与生命科学版）》
CAS
CSCD
北大核心
2005年第3期283-287,共5页
Journal of Zhejiang University:Agriculture and Life Sciences
基金
国家"863"超级稻育种专项(2002AA2070002)
浙江省重点项目("8812"计划)(0011120303).
