摘要
目的 探讨一氧化氮(NO)在慢性病贫血(ACD)发病中的作用,为ACD的防治提供实验依据。方法 用福氏完全佐剂建立ACD的大鼠动物模型,分组给予生理盐水及一氧化氮合酶(NOS)抑制剂L-NAME,观察大鼠后足的肿胀程度,测定血NO、NOS、血红蛋白(Hb)的水平。结果 用L-MAME后关节的炎症反应显著减轻;Hb明显升高,血NO及NOS水平明显降低,经单因素方差分析P<0.01。结论 NO在ACD发病中发挥了重要作用;全身调控NO水平对贫血和关节炎症的改善确有作用。提示研制特异性阻断病理性iNOS和NO的药物应用于临床是治疗ACD的一条新途径。
Objective To investigate the relationship between nitric oxide (NO) and anemia on the basis of establishing the rat model of anemia of chronic disease(ACD). Methods On the basis of traditional adjuvant arthritis rat model lanemia was induced by injecting Freund adjuvant twice so as to enhance the rats immune response , measure the iron .total iron binding capacity(TIBC), trans-ferrin saturation (TS), ferritin(Fn), nitric oxide (NO) and nitric oxide synthase (NOS) in the serum of rats treated with three different ways. Results Varying degree anemia was induced by injecting the Freund adjuvant in adjuvant arthritis rat ,the level SI, TIBC, TS in the serum decreased in ACD rat significantly as compared with the other groups, the level of liver iron content and Fn elevated , which was consistent with the characteristics of iron metabolism disorder in ACD. The degree of arthritic edema and anemia in the rats treated with NOS inhibitor L-NAME was slighter than that of ACD rats , the level of SI, TIBC, TS elevated ( P <0.001).Conclusion NO plays an important role in the disorder of iron metabolism in the ACD rat by establishing of the model of ACD rat successful-ly.
出处
《实用儿科临床杂志》
CAS
CSCD
北大核心
2002年第6期633-634,共2页
Journal of Applied Clinical Pediatrics
关键词
一氧化氮
慢性病
贫血
大鼠
发病
nitric oxide
anemia of chronic disease
iron metabolism
rheumatoid arthritis
