摘要
目的 分析海南地区海口市围产期孕妇无乳链球菌携带状况及其毒力基因分布和药物敏感性。方法 收集海口市中医医院和海南省人民医院门诊部于2019年3月-2021年9月收集的围产期孕妇泌尿生殖道分泌物和直肠拭子标本,对检出的无乳链球菌进行多位点序列(MLST)分型和荚膜多糖基因分型,检测毒力基因分布,进行药物敏感性试验。结果 所有标本经培养分离得到300株无乳链球菌菌株。300株无乳链球菌菌株有237株成功进行MLST分型,共分为19种ST型,主要ST型为ST1、ST19、ST23、ST17、ST12、ST61;共检出Ⅰa、Ⅰb、Ⅱ、Ⅲ、Ⅴ5种型别,主要荚膜多糖型为Ⅲ型;CAMP未检出,hyl B、cyl E、PI-1、PI-2a、PI-2b均检测到阳性株;对青霉素等常见抗菌药物敏感,对红霉素、阿奇霉素等常见抗菌药物耐药。结论 海南地区海口市围产期孕妇主要ST型为ST1、ST19、ST23,主要荚膜多糖型为Ⅲ型,均携带hyl B、cyl E毒力基因,至少携带PI-1、PI-2a、PI-2b其中一个,药物使用推荐青霉素。
OBJECTIVE To investigate the prevalence,virulence genes and drug susceptibility of Streptococcus aga-lactiae strains from perinatal pregnant women in Haikou,Hainan Province.METHODS The urogenital tract secre-tions and rectal swab specimens were collected from the perinatal pregnant women who were treated in outpatient departments of Haikou Hospital of Traditional Chinese Medicine and Hainan Province People's Hospital from Mar 2019 to Sep 2021.Multilocus sequence typing(MLST)and capsular polysaccharide genotyping were carried out for the isolated S.agalactiae strains,the virulence genes were detected,and the drug susceptibility testing was performed.RESULTS Totally 300 strains of S.agalactiae were isolated from all the specimens,237 of which were successfully carried out for the MLST,and 19 types of ST were detected in total,and the major ST types were ST1,ST19,ST23,ST17,ST12 and ST61.Totally 5 typesⅠa,Ⅰb,Ⅱ,m and V were detected,and the typeⅢwas the predominant capsular polysaccharide type.CAMP was not detected;hylB,cylE,PI-1,PI-2a and PI-2 b were detected positive.The strains were sensitive to the commonly used antibiotics such as penicillin but were resistant to erythromycin and azithromycin.CONCLUSION ST1,ST19 and ST23 are dominant among the ST types of the perinatal pregnant women in Haikou,Hainan Province.The typeⅢis the predominant capsular poly-saccharide type.All of the strains carry with the hylB and cylE virulence genes and carry with at least one of PI-1,PI-2a and PI-2b.Penicillin is recommended for clinical use.
作者
张娇珍
陈鑫苹
张新平
许政衡
吴华
ZHANG Jiao-zhen;CHEN Xin-ping;ZHANG Xin-ping;XU Zheng-heng;WU Hua(Haikou Hospital of Traditional Chinese Medicine,Haikou,Hainan 570216,China;不详)
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2024年第5期759-763,共5页
Chinese Journal of Nosocomiology
基金
海南省自然科学基金资助项目(821MS158)。
关键词
无乳链球菌
围产期
毒力基因
药物敏感性
海口市
Streptococcus agalactiae
Perinatal period
Virulence gene
Drug susceptibility testing
Haikou
