摘要
旨在探究云南省笼养食蟹猴与猕猴毕氏肠微孢子虫和环孢子虫的感染及基因型分布情况。从云南省昆明市和玉溪市元江县采集到笼养食蟹猴461份和笼养猕猴72份的新鲜粪便样本,采用巢式PCR方法扩增粪便DNA样本,基于毕氏肠微孢子虫核糖体内转录间隔区和环孢子虫SSU rRNA基因,分别对2种肠道原虫感染进行检测。结果显示:毕氏肠微孢子虫的总感染率为1.5%(8/533)。通过测序分析鉴定出3种已知毕氏肠微孢子虫基因型:D、TypeⅣ和CM1。此外,环孢子虫的总感染率为0.9%(5/533),序列比对仅发现1种环孢子虫基因型。各年龄组、性别、物种和地区间2种原虫的感染率差异无统计学意义(P>0.05)。结果表明,云南地区笼养食蟹猴与猕猴存在较低的毕氏肠微孢子虫和环孢子虫的感染率,该地区存在一定的毕氏肠微孢子虫和环孢子虫传播的潜在风险。本研究结果为我国云南地区笼养食蟹猴与猕猴感染毕氏肠微孢子虫和环孢子虫的防控提供了基础参考资料。
The study aims to investigate the infection and genotype distribution ofEnterocytozoon bieneusi and Cyclospora sp.in captive Macaca fascicularis and Macaca mulatta in Yunnan Province,China.A total of 461 fresh fecal samples from captive M.fascicularis and 72 from captive M.mulatta were collected from Kunming City and Yuanjiang County of Yuxi City,Yunnan Province.A nested PCR method was used to amplify fecal DNA samples,and detection of E.bieneusi was conducted based on the internal transcribed spacer region of the ribosomal RNA gene,while Cyclospora sp.detection was based on the SSU rRNA gene.The results showed that the overall infection rate of E.bieneusi was 1.5%(8/533).Three known genotypes(D,TypeⅣand CM1)of E.bieneusi were identified.Additionally,the overall infection rate of Cyclospora sp.was 0.9%(5/533),and sequence alignment only identified one genotype.There were no statistically significant difference in the infection rates of both two parasites between different age groups,genders,species and regions(P>0.05).These findings indicate a low prevalence ofE.bieneusi and Cyclospora sp.infections in captive M.fascicularis and M.mulatta in Yunnan Province.However,there exists a potential risk of transmission of these parasites in the region.The research results provide fundamental reference data for the prevention and control of E.bieneusi and Cyclospora sp.infections in captive M.fascicularis and M.mulatta in Yunnan Province,China.
作者
马平平
邹扬
牟文杰
张月玥
刘仲藜
张龙
陈利仙
王帅
刘国华
MA Pingping;ZOU Yang;MU Wenjie;ZHANG Yueyue;LIU Zhongli;ZHANG Long;CHEN Lixian;WANG Shuai;LIU Guohua(College of Veterinary Medicine,Hunan Agricultural University,Changsha 410128,China;State Key Laboratory for Animal Disease Control and Prevention/College of Veterinary Medicine of Lanzhou University/Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Lanzhou 730046,China;Kunming Yaling Biology Science and Technology Limited Company,Kunming 650500,China)
出处
《中国兽医科学》
CAS
CSCD
北大核心
2024年第2期233-239,共7页
Chinese Veterinary Science
基金
国家重点研发计划项目(2022YFD1800205)
湖南省自然科学基金联合基金项目(2023JJ5005)
甘肃省杰出青年基金项目(21JR7RA027)。
关键词
云南省
食蟹猴
猕猴
毕氏肠微孢子虫
环孢子虫
Yunnan Province
Macaca fascicularis
Macaca mulatta
Enterocytozoon bieneusi
Cyclospora sp.
