摘要
目的基于网络药理学方法探究“淫羊藿-当归”配伍在治疗骨质疏松症(OP)过程中有效成分和作用机制。方法运用中药系统药理学数据库与分析平台(TCMSP)获取“淫羊藿-当归”配伍有效成分及相对应的预测靶点;通过GeneCards、OMIM、PharmGkb、TTD、DrugBank 5个数据库获取OP靶基因,将以上5个数据库所获取的基因合并取交集,然后将“淫羊藿-当归”和OP的潜在作用靶点取交集,得到“药物-疾病”共有靶点。并用Venny 2.1.0作图工具找出映射,绘制VENN图;通过String 11.5数据库建立蛋白-蛋白相互作用(PPI);通过Cytoscape 3.9.1软件构建相关数据分析网络,筛选出核心靶基因;最后利用DAVID2021数据库对关键靶基因进行富集分析,并用微生信在线绘图软件对已分析的靶基因数据进行GO和京都基因及基因组百科全书(KEGG)通路的可视化绘图。结果获得“淫羊藿-当归”药对涉及22个主要化学成分和129个蛋白靶点,OP相关基因1159个,映射后获得药物与疾病共有靶点74个;PPI分析显示白细胞介素-1B(IL-1B)、V-Jun肉瘤病毒癌基因同源物(JUN)重组蛋白、丝/苏氨酸蛋白激酶(AKT1)等可能是“淫羊藿-当归”药对治疗OP的核心靶点;GO富集分析提示生物学过程主要包括RNA聚合酶II启动子转录的正向调控、基因表达的正向调控等;KEGG通路富集分析提示“淫羊藿-当归”治疗OP作用主要集中在高级糖基化终末产物-受体(AGE-RAGE)、磷脂酰肌醇3激酶/蛋白激酶B(PI3K-Akt)、有丝裂原活化蛋白激酶(MAPK)、白细胞介素-17(IL-17)等信号通路。结论“淫羊藿-当归”配伍可以通过多途径、多靶点治疗OP。
Objective To explore the active components and mechanism of“Epimedium-Angelica”in the treatment of osteoporosis(OP).Methods The Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP)was utilized to obtain the active ingredients and the corresponding predicted targets of“Epimedium-Angelica”.The target genes of OP were obtained from five databases:GeneCards,OMIM,PharmGkb,TTD,and DrugBank.The genes obtained from the above five databases were combined and intersected.Then the potential targets of“Epimedium-Angelica”and OP were intersected to obtain the“drug-disease”common targets.The Venny 2.1.0 mapping tool was used to find out the mapping and draw the venn diagram.Protein-protein interac-tion(PPI)was established by String 11.5 database.The relevant data analysis network was constructed by Cytoscape 3.9.1 software to screen the core target genes.Finally,the key target genes were enriched and analyzed using the DAVID2021 database,and the analyzed target gene data were visualized and mapped for GO and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathways using the Microbiotics online mapping software.Result The Epimedium-Angelica drug pairs involved 24 major chemical components and 129 protein targets,1159 genes associated to osteoporosis.After mapping,74 common targets of drugs and diseases were obtained.PPI analysis revealed that interleukin-1B(interleukin-1B,IL-1B),JUN,filament/threonine protein kinase AKT 1(AKT 1)may be the core targets of“Epimedium-Angelica”drugs for the treatment of OP.GO enrichment analysis suggests that biological processes mainly include the positive regulation of RNA polymerase II promoter transcription and the positive regulation of gene expression.KEGG pathway enrichment analysis suggested that the effects of“Epimedium-Angelica”in the treatment of osteoporosis mainly focused on advanced glycation end product-receptor(AGE-RAGE),phosphatidylinositol 3 kinase/protein kinase B(PI3K-Akt),mitogen-activated protein kinase(mitogen-activated
作者
李志平
李建军
LI Zhiping;LI Jianjun(Second Department of Encephalopathy,Tianshui Hospital of Traditional Chinese Medicine,Tianshui 741000,China)
出处
《湖北民族大学学报（医学版）》
2024年第1期11-17,24,共8页
Journal of Hubei Minzu University(Medical Edition)
基金
国家自然科学基金(82160911)。
关键词
网络药理学
骨质疏松
淫羊藿
当归
作用机制
network pharmacology
osteoporosis
Epimedium
Angelica
mechanism of action
