摘要
目的:多种方法评定大鼠去除双侧卵巢三个月后是否形成明显骨质疏松状态。方法:分别从6月龄SD雌性大鼠假手术对照(Sham)组和卵巢切除骨质疏松(OVX)组各取大鼠8只。将全部大鼠取血后处死。取一侧胫骨、股骨,去净软组织。分别行血生化骨代谢指标检测、MicroCT检查、骨组织计量学测量及组织病理学观察、三点弯曲试验。所得数据经SPSS13.0统计分析。结果:OVX组大鼠ALP、OC较Sham组明显升高,P<0.05。骨小梁数目减少,排列稀疏,骨小梁断裂;骨小梁面积比和体积比、厚度和数目,骨矿含量和骨体积密度等均较Sham组显著降低,骨小梁分离度增加,P<0.05。OVX组弹性载荷与Sham组升高明显,P<0.05;最大应力和弹性应力显著降低P<0.05。结论:经多种方法评定,3月龄SD雌性大鼠切除卵巢3月后,形成明显骨质疏松状态。OVX大鼠,骨量降低,骨小梁结构改变,骨生物力学性能变差。
Objectives： To evaluate the ovariectomized rats after three months as animal model for osteoporosis study. Methods： Eight female Sprague-Dawley （SD） rats aged 24 weeks from sham and OVX group respectivityly were anesthetized. Blood samples were withdrawn to assess biochemical markers of bone metabolism. At sacrifice, the femora and tibia were separated, cleaned of soft tissues. MicroCT scanning, bone histomorphometric analysis and histopathological observation, biomechanical testing were progressed gradually. The data were statistically analyzed by SPSS13.0. Results： Serum ALP activity and osteocalcin levels elevated significantly in OVX group than Sham group （P〈0.05）. Trabecular bone volume and area, trabecular thickness, trabecular number, BMC and BMD of OVX group all were reduced than those of Sham group （P〈0.05）. Trabecular separation increased significantly. Compared with sham group, elastical load of OVX group increased （P〈0.05）. Maximal stress and elastical stress deceased significantly. Conclusions： Evaluated by multi-methods, after three months, the OVX rats are in osteoporotic status. Bone mass is reduced. Trabecular bone architecture has changed. And bone biomechanical performance is worsening.
出处
《中华老年口腔医学杂志》
2009年第4期196-199,234,共5页
Chinese Journal of Geriatric Dentistry
基金
国家自然科学基金资助(项目编号:30772450)
