摘要
目的掌握安徽口岸鼠形动物自然感染巴尔通体的情况。方法2019—2021年在安徽7个口岸范围内用鼠夹、鼠笼捕鼠,从鼠脾中提取DNA,用PCR方法扩增巴尔通体gltA基因。将阳性PCR产物测序,用Mega11软件对序列进行比对分析。结果在安徽7个口岸捕获黑线姬鼠、黄胸鼠、褐家鼠、小家鼠、大足鼠、社鼠和大麝鼩24只,其中黑线姬鼠数量最多,占29.17%。从3只黑线姬鼠中检出巴尔通体核酸,检出率12.50%;其他鼠种均未检出。测序结果显示样本BT438和BT453与未定种的巴尔通体为同一分支,样本BT473与抚远巴尔通体为同一分支。结论安徽口岸地区鼠形动物存在巴尔通体自然感染,且巴尔通体种类多样,应加强口岸鼠类、鼠体表寄生虫等携带病原体的监测。
Objective To investigate the natural infection of Bartonella in rodents at Anhui ports.Methods From 2019 to 2021,the rodents were captured by traps at 7 ports of Anhui.DNA was extracted from spleens samples and Bartonella gltA gene was detected by PCR.The PCR positive products were sequenced,the sequences were compared and analyzed via Mega11.Results A total of 24 rodents only from 3 Apodemus agrarius,Rattus tanezumi,Rattus norvegicus,Mus muscuhus,Rattus nitidus,Niviventer confucianus and Crocidura lasiura were captured at 7 ports of Anhui.Apodemus agrarius accounted for 29.17%of the total.The positive rate of nucleic acid detection of Bartonella was 12.50%(3/24)in the spleens only from 3 Apodemus agrarius.The sequencing results showed that BT438 and BT453 were the same as Uncultured Bartonella strain,and BT473 was the same as Bartonella fuyuanensis strain.Conclusion Natural Bartonella infection existed in rodents at Anhui ports,and there was diversity of Bartonella species.It should be strengthened the surveillance of pathogens carried by rodents and rodent ectoparasites.
作者
许佳
唐海涛
俞保圣
王赛寒
郑迅
郭惠琳
XU Jia;TANG Haitao;YU Baosheng;WANG Saihan;ZHENG Xun;GUO Huilin(Anhui International Travel Healthcare Center(Hefei Customs Port Clinic),Hefei,Anhui 230000,China;不详)
出处
《中国国境卫生检疫杂志》
CAS
2024年第1期38-40,共3页
Chinese Journal of Frontier Health and Quarantine
基金
海关总署科研项目(2023HK058)。
