摘要
【目的】对小麦蔗糖合成酶(sucrose synthase,SUS)基因家族进行鉴定和生物信息学分析,为探究小麦SUS(TaSUS)基因家族的作用机制提供理论参考。【方法】采用生物信息学方法在小麦全基因组上鉴定TaSUS基因家族成员,并对其系统进化关系、染色体位置、基因结构、保守结构域、启动子顺式作用元件和基因表达模式进行分析。【结果】在小麦基因组中共鉴定到分布于14条染色体上的24个TaSUS基因,可分为3个亚组。TaSUS基因含有多个外显子,但部分基因缺失非翻译区结构。TaSUS基因家族成员启动子区域包含45种顺式作用元件,涉及植物生长发育和逆境胁迫响应。大多数TaSUS基因在小麦穗中显著表达,在叶、茎和根中的相对表达量较低。【结论】研究结果有助于了解小麦SUS基因家族的进化,为后期小麦SUS基因家族的生物功能研究奠定理论基础。
[Purpose]To identify the sucrose synthase(SUS)gene family and to analyze the gene bioinformatics,providing a theoretical reference for exploring the mechanism of wheat SUS gene family(TaSUS gene family).[Methods]The TaSUS gene family members were identified on the whole wheat genome by bioinformatics methods.The phylogenetic evolution,chromosome localization,gene structure,conserved domains,cis-acting elements and expression patterns of TaSUS gene family were analyzed.[Results]A total of 24 TaSUS genes distributed on 14 chromosomes were identified from wheat genome,divided into three sub-groups.TaSUS contained multiple exons,but the untranslated region structure of some genes was missing.The promoter region of TaSUS genes contained 45 cis-acting elements,which associated with plant growth and development,and response to stress.Most of TaSUS genes were significantly expressed in wheat spike,but the expression levels in leaves,stems and roots were at low levels.[Conclusion]The result is helpful to understand the evolution of TaSUS gene family,and provides a theoretical basis for further study on the biological function of TaSUS gene family.
作者
孔斌雪
李娜
马靖福
窦佳欣
陈涛
张沛沛
刘媛
杨德龙
KONG Binxue;LI Na;MA Jingfu;DOU Jiaxin;CHEN Tao;ZHANG Peipei;LIU Yuan;YANG Delong(College of Life Science and Technology,Gansu Agricultural University,Lanzhou 730070,China;State Key Laboratory of Aridland Crop Science,Lanzhou 730070,China)
出处
《云南农业大学学报（自然科学版）》
CAS
CSCD
北大核心
2024年第1期1-8,共8页
Journal of Yunnan Agricultural University:Natural Science
基金
中央引导地方科技发展资金项目(23ZYQA0322)
甘肃省农业重点研发计划(21YF5NA089)
甘肃省高等学校产业支撑计划项目(2022CYZC-44)
甘肃省自然科学基金项目(21JR7RA828)
甘肃省高等学校创新基金项目(2021B-125)。
