摘要
【目的】获得外三元猪品种鉴定的有效基因型并建立系统的猪种质鉴定方案,为外三元猪种质鉴定提供思路。【方法】本研究对613头杜洛克猪Illumina 60K芯片、503头长白猪Illumina中芯一号50K芯片及10头大白猪重测序数据进行纯合位点统计分析,筛选获得常染色体上杜洛克猪、长白猪、大白猪的特异性单核苷酸多态性(SNP)位点。以120头不同品系不同品种猪耳组织DNA为模板进行PCR扩增,并测序验证特异性SNP位点,筛选有效的特异性基因位点,通过位点联合分析建立外三元纯种、二元、三元及多元回交猪品种鉴定方案。【结果】通过芯片数据比对共获得6个长白猪特有SNPs位点,8个杜洛克猪特有的SNPs位点,1个大白猪特有SNP位点。测序验证结果显示有效SNPs位点分别为SSC13:57293969 A>G、SSC11:47494227 T>G、SSC6:110653413 T>C和SSC6:110653219 T>C。根据位点突变类型及遗传规律,制定了三位点联合分析的种质鉴定方案,并鉴定出长白纯种猪、多元回交长白猪等多个猪种。【结论】本研究利用猪种特异性SNP位点制定了外三元纯种、二元、三元及多元回交猪品种鉴定方案。试验结果为外三元猪种质鉴定提供参考和技术支撑。
【Objective】The purpose of this experiment was to obtain the effective genotypes for the identification of foreign ternary pig breeds and to establish a systematic identification scheme for the identification of foreign ternary pig germplasm.【Method】In this study,homozygous loci of 613 Duroc pigs Illumina 60K chip,503 Landrace pigs Illumina core No.150K chip and 10 Yarkshire pigs resequencing data were statistically analyzed,and specific single nucleotide polymorphism(SNP)of Duroc,Landrace and Yarkshire pigs were screened.Ear tissue DNA of 120 pigs with different strains and varieties was used as template for PCR amplification and sequencing to verify the specific SNP,and the effective specific gene loci were screened.The identification schemes of foreign ternary purebred,binary,ternary and multiple backcross pigs were established through loci combination analysis.【Result】A total of 6 SNPs specific to Landrace pigs,8 SNPs specific to Duroc pigs and 1 SNP specific to Yarkshire pigs were obtained by comparison of chip data.The effective SNPs were SSC13:57293969 A>G,SSC11:47494227 T>G,SSC6:110653413 T>C and SSC6:110653219 T>C,respectively.According to the mutation types and genetic rules of the loci,the germplasm identification scheme of three-loci joint analysis was developed,and several pig breeds such as Landrace pure breed pigs and multi-backcross Landrace pigs were identified.【Conclusion】In this study,the identification schemes of foreign ternary pure breed,binary,ternary and multiplex backcross pig breeds was developed by pig-specific SNP.The results provided reference and technical support for germplasm identification of foreign ternary pigs.
作者
郑春婷
胡云洋
张春光
叶子源
张琛
罗云燕
秦蕾
彭子璇
于亦飞
冼旺莹
楚菡
洪亮
张建斌
蒲蕾
ZHENG Chunting;HU Yunyang;ZHANG Chunguang;YE Ziyuan;ZHANG Chen;LUO Yunyan;QIN Lei;PENG Zixuan;YU Yifei;XIAN Wangying;CHU Han;HONG Liang;ZHANG Jianbin;PU Lei(Tianjin Key Laboratory of Green Ecological Feed,Tianjin 301800,China;Tianjin Key Laboratory of Agricultural Animal Breeding and Healthy Husbandry,College of Animal Science and Veterinary Medicine,Tianjin Agricultural University,Tianjin 300392,China)
出处
《中国畜牧兽医》
CAS
CSCD
北大核心
2024年第3期1160-1170,共11页
China Animal Husbandry & Veterinary Medicine
基金
天津市科技计划项目(23ZYCGSN00230)
大学生创新创业训练项目(202310061006)
天津市绿色生态饲料重点实验室(TJ202302)
天津市生猪产业技术体系创新团队(ITTPRS2024006)。
关键词
外三元猪
芯片比对
单核苷酸多态性(SNP)
种质鉴定
foreign ternary pig
chip comparison
single nucleotide polymorphism(SNP)
germplasm identification
