摘要
为检测黑色素受体1(melanocortin receptor 1,MC1R)基因型在不同毛色猪种中的分布,研究该基因在猪毛色决定中的地位和作用,本试验使用PCR-SSCP和PCR-RFLP方法对军牧1号白猪、杜洛克猪、西藏小型猪和大白猪的MC1R基因型进行了检测。结果显示,长白猪、大白猪存在nt894insCC和G1197A突变;杜洛克猪存在G668C、C1318T和G1554A突变;西藏小型猪存在C1318T和G1554A突变,而在nt894insCC位点则表现出其他基因型。通过对4个猪种的MC1R基因型的检测,为研究MC1R基因对毛色的作用机理奠定了基础。
For analysis melanocortin receptor 1 (MCIR) genotype in swine spices with different coat color and study the gene functions on swine coat color. This research analysed the genotype of MCIR in Junmu 1 White pigs. Durocs pigs. Tibet mini-pigs and Large White pigs with PCR-SSCP and PCR-RFLP technique. The result showed that Junmu 1 White pigs and Large White pigs presented mutation on nt894insCC and G1l97A site. Durocs pigs presented mutation on G668C. C1318T. and G1554A site. Tibet mini-pigs presented mutation on C1318T and G1554A site, with a different genotype on nt894insCC site. The MCIR genotype result provided a basis for studying MCIR regulation mechanism to swine coat color.
出处
《中国畜牧兽医》
CAS
北大核心
2014年第11期44-49,共6页
China Animal Husbandry & Veterinary Medicine
基金
吉林省科技厅农业处资助项目(20120224)
长春市科技局农业处资助项目(2012231)