摘要
2023年3月,青海省西宁市某牦牛养殖场部分牦牛出现乳房炎,为确定发病原因,从该场采集确诊乳房炎奶牛乳样15份,进行细菌分离、纯化致病菌及PCR鉴定,并采用纸片扩散法检测部分致病菌对抗菌药物的敏感程度。结果发现,从13份乳样中共分离获得23株细菌,分别为无乳链球菌(n=7)、金黄色葡萄球菌(n=8)、大肠埃希氏菌(n=4)、粪肠球菌(n=3)和蜡样芽胞杆菌(n=1)。对无乳链球菌、金黄色葡萄球菌和大肠埃希氏菌进行药敏试验,结果发现,3种病原菌对链霉素、环丙沙星、氧氟沙星、头孢噻呋和头孢拉定高度或中度敏感。研究结果提示,无乳链球菌、金黄色葡萄球菌和大肠埃希氏菌是该场牦牛乳房炎的主要致病菌,且头孢类抗生素和恩诺沙星等可作为首选药物用于该病的防治。
In March 2023,the mastitis disease was observed in a yak farm in Xining City,Qinghai Province.In order to determine the pathogenic agent of this disease,15 milk samples of confirmed mastitis cows were collected from the farm,and bacterial isolation and purification,and PCR identification were performed.The sensitivity of some pathogenic bacteria to antibiotics were analyzed via drug sensitivity test.The results showed that a total of 23 bacterial strains were isolated from 13 milk samples(86.67%,13/15),including Streptococcus agalactis(n=7),Staphylococcus aureus(n=8),Escherichia coli(n=4),Enterococcus faecalis(n=3),and Bacillus cereus(n=1).Drug sensitivity tests were conducted on S.lactis,S.aureus,and E.coli,the results showed that all of the three pathogenic bacteria were highly or moderately sensitive to Streptomycin,Ciprofloxacin,Ofloxacin,Cefotaxime,and Cefradine.The results suggest that S.agalactiae,S.aureus,and E.coli are the main pathogens causing mastitis in the yak farm,and Cephalosporins and Enrofloxacin can be used as the preferred drugs for the prevention and control of the disease.
作者
高明艳
曹振民
屈海珠
张立成
GAO Mingyan;CAO Zhenmin;QU Haizhu;Zhang Licheng(Qinghai Agriculture and Animal Husbandry Technology Vocational and Technical College,Xining 812100,China;Qinghai Animal Disease Prevention and Control Center,Xining 810001,China)
出处
《青海畜牧兽医杂志》
2024年第1期20-23,共4页
Chinese Qinghai Journal of Animal and Veterinary Sciences
关键词
牦牛乳房炎
致病菌
分离鉴定
混合感染
药敏试验
Yak mastitis
Pathogenic bacteria
Isolation and identification
Mixed infection
Drug sensitivity test
