摘要
比较不同产地斑叶兰Goodyeraschlechtendaliana及近缘种植物中两种内酯苷类成分的含量,为斑叶兰药材的质量控制提供参考。采用HPLC-ELSD法,以ChromCore AQ C18(3μm,4.6 mm×250 mm)为色谱柱,超纯水为流动相,等度洗脱;流速0.5 mL·min^(-1),柱温30℃,蒸发光散射检测器雾化温度70℃;载气流速2.5 mL·min^(-1),进样量5μL,测定30批不同产地斑叶兰及近缘种内酯苷类成分含量。结果表明,斑叶兰苷和金线莲苷含量的线性范围均为62.5~1500μg·mL^(-1),相关系数r分别为0.9995、0.9996,精密度、重复性、稳定性的RSD均小于3%;平均加样回收率分别为101.41%和101.16%,RSD分别为1.53%和1.36%。30批斑叶兰及近缘种植物的斑叶兰苷含量在28.31~243.07 mg·g^(-1)之间,平均含量为134.31 mg·g^(-1),存在较大的差异(RSD=38.40%);30批斑叶兰及近缘种植物的金线莲苷含量在0~184.37 mg·g^(-1)之间,平均含量为34.50 mg·g^(-1),也存在较大的差异(RSD=141.30%)。该方法操作简便、快捷、稳定性和重复性良好,具有较好的精密度和准确度,可用于斑叶兰及近缘种内酯苷类成分含量分析。
The contents of lactone glycosides in Goodyera schlechtendaliana and its related species from different habitat were compared in this study to provide reference for the quality control of Goodyera.By HPLC-ELSD method,ChromCore AQ C18(3μm,4.6 mm×250 mm)was used as the chromatographic column,and ultrapure water was used as the mobile phase.The flow rate was 0.5 mL·min^(-1),the column temperature was 30℃,and the atomization temperature of evaporative light scattering detector was 70℃.The flow rate of carrier gas was 2.5 mL·min^(-1),and the sample volume was 5μL.The content of lactone glycosides in 30 batches of G.schlechtendaliana and its related species from different habitat was determined.The linear ranges of goodyeroside A and kinseoside were 62.5-1500μg·mL^(-1)(r=0.9995)and 62.5-1500μg·mL^(-1)(r=0.9996)respectively.The RSD of precision,repeatability and stability were less than 3%,and the average recovery rates were 101.41%and 101.16%respectively.The contents of goodyeroside A in 30 batches of G.schlechtendaliana and its related species were in the range of 28.31-243.07 mg·g^(-1),and the average content of 134.31 mg·g^(-1),which was quite different(RSD=38.40%).The contents of kinseoside in 30 batches of G.schlechtendaliana and its related species were in the range of 0-184.37 mg·g^(-1),and the average content of 34.50 mg·g^(-1),and there were also great differences(RSD=141.30%).The method was simple,rapid,stable and reproducible,with good precision and accuracy,and be used for the content analysis of lactone glycosides in G.schlechtendaliana and its related species.
作者
敖艳霞
冯峰
陈慧玲
梁秀婷
韩玉
吴锦忠
吴岩斌
吴建国
AO Yan-xia;FENG Feng;CHEN Hui-ling;LIANG Xiu-ting;HAN Yu;WU Jin-zhong;WU Yan-bin;WU Jian-guo(College of Pharmacy,Fujian University of Traditional Chinese Medicine,Fuzhou 350122,Fujian China;Department of Pharmacy,Fujian Provincial Governmental Hospital,Fuzhou 350003,Fujian China)
出处
《亚热带植物科学》
CAS
2023年第6期511-517,共7页
Subtropical Plant Science
基金
福建省卫生健康委员会中青年骨干人才培养项目(2021GGA057)。
关键词
斑叶兰
斑叶兰苷
金线莲苷
含量测定
Goodyera schlechtendaliana
goodyeroside A
kinseoside
content determination
