摘要
目的探究妇科门诊患者生殖道感染病原菌定植情况及Wnt/β-连环蛋白(catenin)信号通路表达的诊断价值.方法回顾性分析黑龙江省佳木斯市妇幼保健院2020年3月—2023年3月收治的489例妇科门诊患者的临床资料,根据生殖道感染情况分为感染组77例和非感染组412例;统计妇科门诊患者发生生殖道感染的现状、病原菌定植情况,归纳妇科门诊患者生殖道感染的危险因素,比较两组外周血单个核细胞(PBMC)中Wnt-7a、β-catenin表达水平,采用受试者工作特征(ROC)曲线分析PBMC中Wnt-7a、β-catenin水平单一及联合检测对妇科门诊患者生殖道感染的诊断价值.结果妇科门诊生殖道感染患者共检出病原菌88株,其中革兰阴性菌25株占28.41%,以大肠埃希菌为主;革兰阳性菌29株占32.95%,以凝固酶阴性葡萄球菌为主;真菌12株占13.64%;支原体14株占15.91%;衣原体5株占5.68%;滴虫3株占3.41%;年龄、性行为、无避孕方式、流产史、既往生殖道感染史、文化程度是妇科门诊患者生殖道感染的危险因素(P<0.05);感染组PBMC中Wnt-7a、β-catenin均高于非感染组(P<0.05);PBMC中Wnt-7a、β-catenin联合检测诊断妇科门诊患者生殖道感染的曲线下面积(AUC)高于单一检测(P<0.05),且联合检测的敏感度为81.82%,特异度为83.74%.结论妇科门诊患者生殖道感染病原菌以革兰阳性菌为主;年龄、性行为、无避孕方式、流产史、既往生殖道感染史、文化程度与妇科门诊患者生殖道感染发生密切相关;妇科门诊发生生殖道感染的患者Wnt/β-catenin通路被激活;PBMC中Wnt-7a、β-catenin表达水平两者联合检测可提高妇科门诊患者生殖道感染的诊断价值.
OBJECTIVE To investigate the pathogenic bacteria colonization of reproductive tract infection in gyneco-logical outpatients and the diagnostic value of Wnt/β-catenin signaling pathway.METHODS The clinical data of 489 gynecological outpatients admitted to the Jiamusi Maternal and Child Health Hospital of Heilongjiang Province from Mar.2020 to Mar.2023 were retrospectively analyzed,and the patients were divided into 77 cases of the in-fected group and 412 cases of the non-infected group according to the status of reproductive tract infection.The status and the colonization of pathogenic bacteria in the reproductive tract infection of gynecological outpatients were analyzed,the risk factors of reproductive tract infection in gynecological outpatients were summarized,the expression levels of Wnt-7a andβ-catenin in peripheral blood mononuclear cells(PBMC)of the non-infected and the infected groups were compared,and receiver operating characteristic(ROC)curve was used to analyze the di-agnostic value of single and combined detection of Wnt-7a andβ-catenin levels in PBMC in gynecological outpa-tients with reproductive tract infection.RESULTS A total of 88 strains of pathogenic bacteria were detected in pa-tients with reproductive tract infection in gynecological clinic,of which 25 strains of gram-negative bacteria ac-counted for 28.41%,predominating by Escherichia coli.29 strains of Gram-positive bacteria accounted for 32.95%,predominating by coagulase negative Staphylococcus.12 strains of fungi accounted for 13.64%.Myco-plasma 14 strains accounted for 15.91%.5 strains of Chlamydia accounted for 5.68%.Three strains of Trichomonas accounted for 3.41%.Age,sexual behavior,lack of contraception,history of abortion,history of reproductive tract infection and education level were the risk factors of reproductive tract infection in gynecological outpatients(P<0.05).The levels of Wnt-7a andβ-catenin in PBMC in the infected group were higher than those in the non-infected group(P<0.05).The area under the curve(AUC)of the com
作者
曲春尧
孙健
杨丽杰
肖晓超
李璐
QU Chun-yao;SUN Jian;YANG Li-jie;XIAO Xiao-chao;LI Lu(Jiamusi Maternal and Child Health Hospital,Jiamusi,Heilongjiang 154000,China)
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2024年第4期552-556,共5页
Chinese Journal of Nosocomiology
基金
黑龙江省卫生健康委科研基金资助项目(20210505020199)。
关键词
妇科门诊
生殖道感染
病原菌
危险因素
Wnt/β-连环蛋白
受试者工作特征曲线
诊断价值
Gynecological clinic
Reproductive tract infections
Pathogenic bacteria
Risk factor
Wnt/β-catenin
Receiver operating characteristic curve
Diagnostic value
