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天麻块茎腐烂病病原鉴定及其致病性测定

Taxonomic identification and pathogenicity determination of the causal agent for tuber rot of Gastrodia elata
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摘要 0 引言天麻(Gastrodia elata Blume)属兰科(Orchidaceae),是一种异养型多年生草本植物,其种子需与萌发菌(Mycena osmundicola)共生,其生长依赖蜜环菌(Armillaria mellea)提供营养[1]。天麻为我国传统名贵中药材,药食两用。天麻块茎腐烂病是制约天麻规模化发展的瓶颈。依据天麻花、茎的颜色特征可将天麻划分为5种生态型:红天麻(G. elata f. elat)、绿天麻(G.elataf.viridis)。 Gastrodia elata is a perennial symbiotic heterotrophic herb of Orchidaceae,and it is an authentic medicinal and edible plant mainly produced in China.In 2021,tuber rot of G.elata seriously occurred in Yunnan province of China.To identify the causal agent of this disease,a fungus was isolated,and identified by morphological characterization and analyses of the nucleotide sequences of ITS-5.8S rDNA and RPB2(for RNA polymerase II second largest subunit).A pathogenicity test was performed to fulfill Koch's postulates,and the inoculated isolate was re-isolated from the infected tubers.The results showed that tuber rot on G.elata caused by Verticillium albo-atrum in China.This is the first report of orchidaceous species infected by V.albo-atrum,and the result is helpful for development of the effective control strategies and methods.
作者 苏建云 唐晶莹 董鲜 董家红 季鹏章 张磊 SU Jianyun;TANG Jingying;DONG Xian;DONG Jiahong;JI Pengzhang;ZHANG Lei(College of Chinese Materia Medica and Yunnan Key Laboratory of Southern Medicinal Utilization,Yunnan University of Chinese Medicine,Kunming 650500,China)
出处 《植物病理学报》 CAS CSCD 北大核心 2023年第6期1241-1245,共5页 Acta Phytopathologica Sinica
基金 云南省科技厅基础研究专项计划(202101AT070245) 云南省科技厅、云南中医药大学应用基础研究联合专项基础研究计划(202101AZ070001-054) 云南省科技人才和平台计划(202105AG070012) “云药之乡西北部片区产业技术创新及应用”子课题“云药之乡主要中药材良种繁育技术创新及应用”(202102AA310045-02) 云南中医药大学高层次人才科研项目(30270104868、30270104870)。
关键词 名贵中药材 块茎腐烂病 致病性测定 蜜环菌 萌发菌 病原鉴定 颜色特征 药食两用 Gastrodia elata tuber rot Verticillium albo-atrum
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